Overexpression of IRM1 enhances resistance to aphids in Arabidopsis thaliana.
Overexpression of IRM1 enhances resistance to aphids in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0070914
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Vosman B
中科院分区：
文献类型：
--
作者：
Chen X;Zhang Z;Visser RG;Broekgaarden C;Vosman B
蚜虫是一种通过移除韧皮部汁液对作物造成直接损害的昆虫，但更重要的是，它们传播毁灭性的病毒。蚜虫利用其复杂的口器（即口针）从寄主植物的韧皮部筛元件中取食。为了鉴定影响宿主植物对蚜虫抗性的基因，我们先前筛选了拟南芥激活标签突变体集合。在这样的突变体中，标记的基因通过与天然启动子相邻的强35 S增强子过表达，导致显性功能获得表型。我们以前确定了几个这些突变体上的蚜虫桃蚜表现出减少的人口发展与野生型相比。在本研究中，我们表明，基因的表型负责的突变体之一是At 5g 65040，并命名为该基因增加抗桃蚜1（IRM 1）。克隆的IRM 1基因的过表达赋予了与原始突变体相同的表型。相反，IRM 1基因敲除突变体促进蚜虫种群发展相比，野生型。我们进行了电穿透图分析，以研究蚜虫的探测和进食行为是如何影响植物，无论是过表达IRM 1或包含在这个基因敲除突变。EPG结果表明，蚜虫在向韧皮部的过度表达线上移动时遇到了抗性因子。这种抗性机制也影响到其他蚜虫物种，并建议机械性质。有趣的是，IRM 1表达响应蚜虫攻击的遗传变异被观察到。在蚜虫攻击后，IRM 1的表达最初（6小时后）在生态型Wassilewskija中被诱导，随后被抑制。在哥伦比亚-0中，在感染开始后6小时，IRM 1表达已经被抑制。IRM 1过表达在A. thaliana与plant植物growth生长.
Aphids are insects that cause direct damage to crops by the removal of phloem sap, but more importantly they spread devastating viruses. Aphids use their sophisticated mouthpart (i.e. stylet) to feed from the phloem sieve elements of the host plant. To identify genes that affect host plant resistance to aphids, we previously screened an Arabidopsis thaliana activation tag mutant collection. In such mutants, tagged genes are overexpressed by a strong 35S enhancer adjacent to the natural promoter, resulting in a dominant gain-of-function phenotype. We previously identified several of these mutants on which the aphid Myzus persicae showed a reduced population development compared with wild type. In the present study we show that the gene responsible for the phenotype of one of the mutants is At5g65040 and named this gene Increased Resistance to Myzus persicae 1 (IRM1). Overexpression of the cloned IRM1 gene conferred a phenotype identical to that of the original mutant. Conversely, an IRM1 knockout mutant promoted aphid population development compared to the wild type. We performed Electrical Penetration Graph analysis to investigate how probing and feeding behaviour of aphids was affected on plants that either overexpressed IRM1 or contained a knockout mutation in this gene. The EPG results indicated that the aphids encounter resistance factors while reaching for the phloem on the overexpressing line. This resistance mechanism also affected other aphid species and is suggested to be of mechanical nature. Interestingly, genetic variation for IRM1 expression in response to aphid attack was observed. Upon aphid attack the expression of IRM1 was initially (after 6 hours) induced in ecotype Wassilewskija followed by suppression. In Columbia-0, IRM1 expression was already suppressed six hours after the start of the infestation. The resistance conferred by the overexpression of IRM1 in A. thaliana trades off with plant growth.
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影响因子：
7.3
作者：
Divol, Fanchon;Vilaine, Francoise;Dinant, Sylvie
通讯作者：
Dinant, Sylvie
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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