Neutrophil-mediated damage to human gingival epithelial cells.
Neutrophil-mediated damage to human gingival epithelial cells.
复制标题
中性粒细胞介导的人牙龈上皮细胞损伤。
DOI：
10.1111/j.1600-0765.1992.tb02088.x
复制
发表时间：
1992
影响因子：
3.5
通讯作者：
Oda,D
中科院分区：
文献类型：
--
作者：
Altman,LC;Baker,C;Fleckman,P;Luchtel,D;Oda,D
多形核白细胞（PMNs）参与炎症性牙龈炎和牙周炎的发病机制。为了进一步研究中性粒细胞在介导牙龈损伤中的作用，我们将这些细胞与人牙龈上皮细胞单层体外共培养。扫描电子显微镜显示上皮细胞是同质的，SDS-PAGE/免疫印迹分析鉴定了角蛋白K3、K13和K.6/16对的存在，这证实了细胞的口腔来源。牙龈细胞的损伤通过扫描电子显微镜和细胞脱落和细胞溶解的测量来确定。未受刺激的PMN产生最小的溶解或脱离，但由佛波醇肉豆蔻酸酯乙酸酯刺激的PMN产生显著的上皮细胞脱离而不溶解，这是时间和PMN剂量依赖性的。活化的中性粒细胞的上清液也同样有效，表明介质是一种稳定的可溶性物质。弹性蛋白酶和组织蛋白酶G，两种中性蛋白酶的PMN起源，产生时间和浓度依赖性的牙龈上皮细胞的脱离，这表明这些酶可能介导这种形式的损伤。在其他研究中，将牙龈上皮细胞暴露于PMN骨髓氧化酶（MPO）、氯化物和葡萄糖加葡萄糖氧化酶（GO）作为过氧化氢（H2 O2）生成系统。由该系统产生的有毒氧引起上皮靶细胞的溶解，这取决于孵育的持续时间和MPO和GO的浓度。MPO的抑制剂叠氮和H_2O_2的清除剂过氧化氢酶抑制该系统的裂解活性。牙龈上皮细胞与激活的中性粒细胞共培养的扫描电子显微镜照片显示，从平板表面的细胞升降，而MPO系统攻击的靶细胞显示广泛的细胞膜损伤。这些研究表明，激活的中性粒细胞引起牙龈上皮细胞的非溶解性脱落损伤，这可能是由颗粒中性蛋白酶消化其细胞外基质介导的。此外，PMN MPO能够产生毒性氧物质，其可以溶解这些上皮细胞。总的来说，这些行为可能对牙龈上皮的功能和完整性产生深远的不良影响。
Polymorphonuclear leukocytes (PMNs) have been implicated in the pathogenesis of inflammatory gingivitis and periodontitis. To further study the role of PMNs in mediating gingival injury, we cocultured these cellsin vitrowith monolayers of human gingival epithelial cells. Scanning electron microscopy revealed that the epithelial cells were homogeneous and SDS‐PAGE/immunoblot analysis identified the presence of keratins K3, K13 and the K.6/16 pair which authenticated the oral origin of the cells. Injury to the gingival cells was determined by scanning electron microscopy and measurement of cell detachment and cytolysis. Unstimulated PMNs produced minimal lysis or detachment, but PMNs stimulated by phorbol myristate acetate produced marked epithelial cell detachment without lysis, which was time‐ and PMN‐dose‐dependent. Supernatants of activated PMNs were similarly effective, indicating that the mediator was a stable soluble substance. Elastase and cathepsin G, two neutral proteases of PMN origin, produced time‐ and concentration‐dependent detachment of gingival epithelial cells, suggesting that these enzymes may mediate this form of injury. In other studies, gingival epithelial cells were exposed to PMN myeloper‐oxidase (MPO), chloride and glucose plus glucose oxidase (GO) as a hydrogen peroxide (H2O2) generating system. The toxic oxygen species produced by this system caused lysis of the epithelial targets which was dependent on the duration of incubation and the concentrations of MPO and GO. Azide, an inhibitor of MPO, and catalase, a scavenger of H2O2, inhibited the lytic activity of this system. Scanning electron micrographs of gingival epithelial cells cocultured with activated PMNs showed lifting of the cells from the plating surface, while target cells attacked by the MPO system revealed extensive damage of cell membranes. These studies indicate that activated PMNs cause nonlytic detachment injury to gingival epithelial cells which may be mediated by digestion of their extracellular matrix by granule neutral proteases. Furthermore, PMN MPO is capable of generating toxic oxygen species which can lyse these epithelial cells. Collectively, these actions could have profound adverse effects on the function and integrity of the gingival epithelium.
登录
查看更多内容
慢性成人牙周炎、局部青少年牙周炎和牙龈炎患者以及健康对照受试者的沟液中的胶原蛋白溶解活性。
影响因子：
3.5
作者：
Benardo Villela;R. Cogen;A. Bartolucci;H. Birkedal‐Hansen
通讯作者：
H. Birkedal‐Hansen
实验性人类牙龈炎。
影响因子：
4.3
作者：
L. Friedman;J. M. Klinkhamer
通讯作者：
J. M. Klinkhamer
各种形式的牙周炎患者白细胞趋化性异常。
影响因子：
3.5
作者：
Altman,LC;Page,RC;Vandesteen,GE;Dixon,LI;Bradford,C
通讯作者：
Bradford,C
影响因子：
3.5
作者：
KUNIMATSU, K;YAMAMOTO, K;KATO, I
通讯作者：
KATO, I
过氧化氢会对暴露于氧自由基的人体成纤维细胞造成致命伤害。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Simon,RH;Scoggin,CH;Patterson,D
通讯作者：
Patterson,D