Isolation and characterization of polymorphic microsatellite markers in the fish Garra orientalis (oriental sucking barb)

Isolation and characterization of polymorphic microsatellite markers in the fish Garra orientalis (oriental sucking barb)
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东方吸嘴鱼多态性微卫星标记的分离和表征

DOI:
10.1007/s12686-012-9776-5
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发表时间:
2013-03
影响因子:
1.1
通讯作者:
Yang, Jin-Quan
Yang, Jin-Quan
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Zeng, Zhen;Liu, Ang-Yu;Tang, Wen-Qiao;Yang, Jin-Quan

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摘要开发了14个东方墨头鱼多态性微卫星标记。对采自海南岛万泉河的23个个体进行了检测。等位基因数(NA)、多态信息含量、观察杂合度和期望杂合度分别为8 ~ 25、0.6788 ~ 0.9342、0.5652 ~ 1.0000和0.7179 ~ 0.9585。有4个位点由于杂合子缺失而偏离了Hardy-Weinberg平衡。这些标记可用于研究该物种的遗传多样性、种群结构、保护和合理利用。
Abstract14 Polymorphic microsatellite markers were developed in the Garra orientalis. These new markers were tested in 23 individuals collected from the Wanquan River in the Hainan Island. The number of alleles (NA), polymorphism information content, observed and expected heterozygosity per locus ranged from 8 to 25, 0.6788 to 0.9342, 0.5652 to 1.0000 and 0.7179 to 0.9585, respectively. Four loci are deviated from Hardy–Weinberg equilibrium due to the heterozygote deficiency. These markers can be used to study genetic diversity, population structure, conservation and rational utilization of this species.
使用基于 PCR 的微卫星阵列分离 (PIMA) 从 Squalidus argentatus 中分离多态性微卫星位点
DOI: 10.3390/ijms12095666
发表时间: 2011
影响因子: 5.6
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