Polymorphic microsatellite loci isolated from the Squalidus argentatus using PCR-Based Isolation of Microsatellite Arrays (PIMA).
Polymorphic microsatellite loci isolated from the Squalidus argentatus using PCR-Based Isolation of Microsatellite Arrays (PIMA).
复制标题
使用基于 PCR 的微卫星阵列分离 (PIMA) 从 Squalidus argentatus 中分离多态性微卫星位点
DOI：
10.3390/ijms12095666
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发表时间：
2011
影响因子：
5.6
通讯作者：
Yang JQ
中科院分区：
文献类型：
--
作者：
Sun Y;Lin HD;Tang WQ;Ju YM;Liu ZZ;Liu D;Yang JQ
Squalidus argentatus (Sauvage and Dabry de Thiersant 1874) 是一种小型淡水鱼，分布于中国大陆、海南岛和台湾。银鳅的数量急剧下降，可能是由于近期的过度捕捞和水污染。为鲤鱼 S. argentatus 开发了 11 个多态性微卫星标记。这些新标记在从长江和钱塘江采集的 43 个个体上进行了测试。两个群体中等位基因的数量、每个基因座观察到的杂合性和预期杂合性的范围分别为 3 至 14、0.333 至 0.954 和 0.480 至 0.928。由于杂合子缺陷，只有两个基因座显着偏离 Hardy-Weinberg 预期。这些基因座的成对比较之间没有检测到显着的连锁不平衡。这些多态性微卫星位点将使我们能够在未来研究该物种的遗传变异、种群结构和保护遗传学。
Squalidus argentatus (Sauvage and Dabry de Thiersant 1874) is a small-sized freshwater fish which is distributed in Mainland China, Hainan Island and Taiwan. The populations of S. argentatus have dropped sharply probably due to overharvesting and water pollution recently. Eleven polymorphic microsatellite markers were developed for the cyprinid fish S. argentatus. These new markers were tested on 43 individuals collected from Yangtze River and Qiantang River. The number of alleles, observed and expected heterozygosity per locus, in two populations ranged from 3 to 14, from 0.333 to 0.954 and from 0.480 to 0.928, respectively. Only two loci are significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency. No significant linkage disequilibrium was detected between the pairwise comparisons of these loci. These polymorphic microsatellite loci will enable us to study the genetic variation, population structure, and conservation genetics of this species in the future.
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影响因子：
7.7
作者：
Excoffier, Laurent;Lischer, Heidi E. L.
通讯作者：
Lischer, Heidi E. L.
DOI：
10.1111/j.1471-8286.2004.00684.x
发表时间：
2004-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Van Oosterhout, C;Hutchinson, WF;Shipley, P
通讯作者：
Shipley, P
影响因子：
14.9
作者：
CIFARELLI, RA;GALLITELLI, M;CELLINI, F
通讯作者：
CELLINI, F
影响因子：
2.2
作者：
Chiang, Tzen-Yuh;Lee, Teh-Wang;Lin, Hung-Du
通讯作者：
Lin, Hung-Du
影响因子：
4.9
作者：
Lunt, DH;Hutchinson, WF;Carvalho, GR
通讯作者：
Carvalho, GR