A Meloidogyne graminicola C-type lectin, Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism.

A Meloidogyne graminicola C-type lectin, Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism.
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根结线虫 C 型凝集素 Mg01965 分泌到宿主质外体中,抑制植物防御并促进寄生

DOI:
10.1111/mpp.12759
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发表时间:
2019-03
影响因子:
4.9
通讯作者:
Gheysen G
Gheysen G
中科院分区:
农林科学1区
文献类型:
--
作者:
Zhuo K;Naalden D;Nowak S;Xuan Huy N;Bauters L;Gheysen G

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C型凝集素(CTL)是一类多功能蛋白质,在线虫中大量存在。本研究进一步分析了一个CTL基因,Mg01965,该基因在根结线虫的亚腹腺中表达,特别是在草生根结线虫的第二阶段幼虫中。在体外RNA干扰靶向Mg01965的幼虫显着降低其感染宿主植物根部的能力。免疫定位结果表明,Mg 01965是分泌到根在早期寄生阶段,并积累在质外体。Mg01965在本氏烟草中的瞬时表达并将其靶向到质外体抑制了flg22引发的活性氧簇的爆发。CTL Mg01965抑制宿主质外体中的植物先天免疫,促进线虫在感染早期阶段的寄生。
C‐type lectins (CTLs), a class of multifunctional proteins, are numerous in nematodes. One CTL gene, Mg01965, shown to be expressed in the subventral glands, especially in the second‐stage juveniles of the root‐knot nematode Meloidogyne graminicola, was further analysed in this study. In vitro RNA interference targeting Mg01965 in the preparasitic juveniles significantly reduced their ability to infect host plant roots. Immunolocalizations showed that Mg01965 is secreted by M. graminicola into the roots during the early parasitic stages and accumulates in the apoplast. Transient expression of Mg01965 in Nicotiana benthamiana and targeting it to the apoplast suppressed the burst of reactive oxygen species triggered by flg22. The CTL Mg01965 suppresses plant innate immunity in the host apoplast, promoting nematode parasitism in the early infection stages.
DOI: 10.1016/j.parint.2009.08.011
发表时间: 2009-12
影响因子: 1.9
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