A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system.
A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system.
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DOI：
10.1111/nph.13701
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Liao J
中科院分区：
文献类型：
--
作者：
Lin B;Zhuo K;Chen S;Hu L;Sun L;Wang X;Zhang LH;Liao J
有证据表明，植物寄生线虫可以分泌效应子来干扰宿主的免疫反应，但这些效应子如何征服宿主的免疫反应仍然是未知的。在这里，我们描述了一种新的效应，MjTTL5，可以抑制植物免疫反应。免疫定位和转录分析表明，MjTTL5特异性表达于爪哇根结线虫的亚腹腺内，并在线虫的早期寄生阶段上调。表达MjTTL5的转基因拟南芥系比野生型植物显著更易受爪哇根结线虫感染，反之亦然，植物中MjTTL5沉默显著增加了植物对爪哇根结线虫的抗性。酵母双杂交、免疫共沉淀和双分子荧光互补分析表明，MjTTL 5与拟南芥铁氧还蛋白：硫氧还蛋白还原酶催化亚基（AtFTRc）特异性相互作用，AtFTRc是宿主抗氧化系统的关键组分。AtFTRc的表达由爪哇分枝杆菌感染诱导。AtFTRc和MjTTL之间的相互作用可以显著提高寄主的活性氧清除活性，从而抑制植物的基础防御能力，减弱寄主对线虫侵染的抗性。我们的研究结果表明，宿主铁氧还蛋白：硫氧还蛋白系统可以巧妙地利用由M. javanica，揭示了一种新的机制，利用植物寄生线虫征服植物天然免疫，从而促进寄生。
Evidence is emerging that plant‐parasitic nematodes can secrete effectors to interfere with the host immune response, but it remains unknown how these effectors can conquer host immune responses. Here, we depict a novel effector, MjTTL5, that could suppress plant immune response. Immunolocalization and transcriptional analyses showed that MjTTL5 is expressed specifically within the subventral gland of Meloidogyne javanica and up‐regulated in the early parasitic stage of the nematode. Transgenic Arabidopsis lines expressing MjTTL5 were significantly more susceptible to M. javanica infection than wild‐type plants, and vice versa, in planta silencing of MjTTL5 substantially increased plant resistance to M. javanica. Yeast two‐hybrid, coimmunoprecipitation and bimolecular fluorescent complementation assays showed that MjTTL5 interacts specifically with Arabidopsis ferredoxin : thioredoxin reductase catalytic subunit (AtFTRc), a key component of host antioxidant system. The expression of AtFTRc is induced by the infection of M. javanica. Interaction between AtFTRc and MjTTL could drastically increase host reactive oxygen species‐scavenging activity, and result in suppression of plant basal defenses and attenuation of host resistance to the nematode infection. Our results demonstrate that the host ferredoxin : thioredoxin system can be exploited cunningly by M. javanica, revealing a novel mechanism utilized by plant–parasitic nematodes to subjugate plant innate immunity and thereby promoting parasitism.
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影响因子：
30.3
作者：
Cheng W;Munkvold KR;Gao H;Mathieu J;Schwizer S;Wang S;Yan YB;Wang J;Martin GB;Chai J
通讯作者：
Chai J
影响因子：
4.3
作者：
Balsera, Monica;Uberegui, Estefania;Buchanan, Bob B.
通讯作者：
Buchanan, Bob B.
影响因子：
7.2
作者：
Barcala, Marta;Garcia, Alejandra;Escobar, Carolina
通讯作者：
Escobar, Carolina
影响因子：
64.8
作者：
Asai, T;Tena, G;Sheen, J
通讯作者：
Sheen, J
影响因子：
56.9
作者：
de Jonge, Ronnie;van Esse, H. Peter;Thomma, Bart P. H. J.
通讯作者：
Thomma, Bart P. H. J.