Wolbachia infections in Anopheles gambiae cells: transcriptomic characterization of a novel host-symbiont interaction.
Wolbachia infections in Anopheles gambiae cells: transcriptomic characterization of a novel host-symbiont interaction.
复制标题
沃尔巴氏菌感染冈比亚细胞：新型宿主 - 伴侣相互作用的转录组表征。
DOI：
10.1371/journal.ppat.1001296
复制
发表时间：
2011-02
期刊：
影响因子：
6.7
通讯作者：
Rasgon JL
中科院分区：
文献类型：
--
作者：
Hughes GL;Ren X;Ramirez JL;Sakamoto JM;Bailey JA;Jedlicka AE;Rasgon JL
正在研究内共生细菌沃尔巴克氏体作为几种重要媒介昆虫物种的潜在控制剂。最近的研究表明，沃尔巴克氏体可以保护昆虫宿主免受各种病原体的侵害，从而减少寄生虫和病毒的传播。已经提出，受Wolbachia感染的昆虫的载体能力受损是由于宿主先天免疫系统或代谢竞争的上调。传播人类疟疾寄生虫的按蚊在自然界中从未被发现携带沃尔巴克氏体。虽然可以在按蚊中建立短暂的体细胞感染，但尽管进行了多次尝试，仍没有开发出稳定的人工感染按蚊品系。然而，培养的按蚊细胞可以稳定地感染多种沃尔巴克氏体菌株，例如来自白纹伊蚊的wAlbB、来自拟果蝇的wRi和来自黑腹果蝇的wMelPop。受感染的细胞系提供了一个适合的系统，以调查沃尔巴克氏体-按蚊相互作用的情况下，受感染的蚊子株。我们使用了Affychip基因芯片微阵列来研究wAlbB和wRi感染对培养的按蚊Sua 5 B细胞的转录组的影响，并且对于一个基因子集，使用定量PCR来验证体细胞感染的按蚊的结果。沃尔巴克氏体感染对该细胞系中的基因表达具有显著的菌株特异性影响，总共调节了近700个基因，代表了不同的功能类别。非常引人注目的是，感染导致许多免疫、压力和解毒相关转录物的显著下调。这与在其他昆虫宿主中观察到的免疫基因的诱导形成鲜明对比。我们还确定了可能参与沃尔巴克氏体诱导的生殖和致病表型的基因。体细胞感染的蚊子对培养的细胞也有类似的反应。这些数据表明，沃尔巴克氏体对培养细胞中的按蚊基因表达具有深刻而独特的影响，并对沃尔巴克氏体诱导的表型的机制理解和控制疟疾的潜在新策略具有重要意义。 沃尔巴克氏体是一种感染许多昆虫物种的细菌，但不会感染按蚊。这些蚊子传播疟原虫寄生虫，导致人类疟疾。埃及伊蚊中的沃尔巴克氏体感染降低了它们传播包括病毒、线虫和鸟疟疾寄生虫在内的多种病原体的能力。沃尔巴克氏体诱导的蚊子先天免疫系统的刺激被认为是赋予这种病原体干扰的机制。由于不存在感染沃尔巴克氏体的按蚊品系，我们使用感染的培养按蚊细胞来检测感染对按蚊基因表达的影响。沃尔巴克氏体对按蚊基因表达有深远的影响。在其他研究中，沃尔巴克氏体调控的许多基因都影响了蚊子体内的疟原虫水平，但有趣的是，与其他蚊子相反，许多宿主基因被抑制而不是被诱导。
The endosymbiotic bacterium Wolbachia is being investigated as a potential control agent in several important vector insect species. Recent studies have shown that Wolbachia can protect the insect host against a wide variety of pathogens, resulting in reduced transmission of parasites and viruses. It has been proposed that compromised vector competence of Wolbachia-infected insects is due to up-regulation of the host innate immune system or metabolic competition. Anopheles mosquitoes, which transmit human malaria parasites, have never been found to harbor Wolbachia in nature. While transient somatic infections can be established in Anopheles, no stable artificially-transinfected Anopheles line has been developed despite numerous attempts. However, cultured Anopheles cells can be stably infected with multiple Wolbachia strains such as wAlbB from Aedes albopictus, wRi from Drosophila simulans and wMelPop from Drosophila melanogaster. Infected cell lines provide an amenable system to investigate Wolbachia-Anopheles interactions in the absence of an infected mosquito strain. We used Affymetrix GeneChip microarrays to investigate the effect of wAlbB and wRi infection on the transcriptome of cultured Anopheles Sua5B cells, and for a subset of genes used quantitative PCR to validate results in somatically-infected Anopheles mosquitoes. Wolbachia infection had a dramatic strain-specific effect on gene expression in this cell line, with almost 700 genes in total regulated representing a diverse array of functional classes. Very strikingly, infection resulted in a significant down-regulation of many immune, stress and detoxification-related transcripts. This is in stark contrast to the induction of immune genes observed in other insect hosts. We also identified genes that may be potentially involved in Wolbachia-induced reproductive and pathogenic phenotypes. Somatically-infected mosquitoes had similar responses to cultured cells. The data show that Wolbachia has a profound and unique effect on Anopheles gene expression in cultured cells, and has important implications for mechanistic understanding of Wolbachia-induced phenotypes and potential novel strategies to control malaria. Wolbachia are bacteria that infect many insect species, but do not infect Anopheles mosquitoes. These mosquitoes transmit Plasmodium parasites, which cause malaria in humans. Wolbachia infection in Aedes aegypti mosquitoes reduces their ability to transmit diverse pathogens including viruses, nematodes and bird malaria parasites. Wolbachia-induced stimulation of the mosquito's innate immune system has been suggested as a mechanism conferring this pathogen interference. Since no Wolbachia-infected Anopheles mosquito strain exists, we used infected cultured Anopheles cells to examine the effect of infection on Anopheles gene expression. Wolbachia had a profound influence on Anopheles gene expression. Many of the genes regulated by Wolbachia have been seen in other studies to influence Plasmodium levels in mosquitoes, but interestingly and in contrast to other mosquitoes, many of the host genes were suppressed rather than induced.
登录
查看更多内容
影响因子：
6.7
作者：
Dong Y;Aguilar R;Xi Z;Warr E;Mongin E;Dimopoulos G
通讯作者：
Dimopoulos G
影响因子：
4.4
作者：
Dana AN;Hong YS;Kern MK;Hillenmeyer ME;Harker BW;Lobo NF;Hogan JR;Romans P;Collins FH
通讯作者：
Collins FH
影响因子：
3.7
作者：
Brennan LJ;Keddie BA;Braig HR;Harris HL
通讯作者：
Harris HL
影响因子：
6.7
作者：
Kambris Z;Blagborough AM;Pinto SB;Blagrove MS;Godfray HC;Sinden RE;Sinkins SP
通讯作者：
Sinkins SP
影响因子：
14.9
作者：
Barrett T;Troup DB;Wilhite SE;Ledoux P;Rudnev D;Evangelista C;Kim IF;Soboleva A;Tomashevsky M;Marshall KA;Phillippy KH;Sherman PM;Muertter RN;Edgar R
通讯作者：
Edgar R