Synthesis and characterization of hybrid hyaluronic acid-gelatin hydrogels.
Synthesis and characterization of hybrid hyaluronic acid-gelatin hydrogels.
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DOI：
10.1021/bm3019856
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发表时间：
2013-04-08
影响因子：
6.2
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Camci-Unal, Gulden;Cuttica, Davide;Annabi, Nasim;Demarchi, Danilo;Khademhosseini, Ali
仿生杂化水凝胶在组织工程和再生医学中引起了广泛的兴趣。透明质酸（HA）和明胶（水解胶原）是天然来源的聚合物，在生理条件下可生物降解。此外，胶原和HA是大多数组织（例如心血管、软骨、神经）中细胞外基质（ECM）的主要成分。当用作混合材料时，HA-明胶水凝胶可以模拟天然组织的ECM。尽管HA-明胶混杂水凝胶是有前途的仿生基底，但文献中尚未彻底表征其材料性质。在此，我们通过使用不同浓度的HA和明胶生成具有可调物理和生物性质的混合水凝胶。研究了制备的水凝胶的物理性能，包括溶胀比、降解和机械性能。此外，还评估了二维和三维（2D和3D）培养条件下的体外细胞反应。结果表明，在HA甲基丙烯酸酯（HAMA）中加入明胶甲基丙烯酸酯（GelMA）可促进细胞在杂化水凝胶中的铺展。此外，混合水凝胶显示出显着改善的机械性能相比，其单一组分类似物。HAMA-GelMA水凝胶表现出显著的可调谐性行为，可能用于心血管组织工程应用。
Biomimetic hybrid hydrogels have generated broad interest in tissue engineering and regenerative medicine. Hyaluronic acid (HA) and gelatin (hydrolyzed collagen) are naturally derived polymers and biodegradable under physiological conditions. Moreover, collagen and HA are major components of the extracellular matrix (ECM) in most of the tissues (e.g. cardiovascular, cartilage, neural). When used as a hybrid material, HA-gelatin hydrogels may enable mimicking the ECM of native tissues. Although HA-gelatin hybrid hydrogels are promising biomimetic substrates, their material properties have not been thoroughly characterized in the literature. Herein, we generated hybrid hydrogels with tunable physical and biological properties by using different concentrations of HA and gelatin. The physical properties of the fabricated hydrogels including swelling ratio, degradation, and mechanical properties were investigated. In addition, in vitro cellular responses in both two and three dimensional (2D and 3D) culture conditions were assessed. It was found that the addition of gelatin methacrylate (GelMA) into HA methacrylate (HAMA) promoted cell spreading in the hybrid hydogels. Moreover, the hybrid hydrogels showed significantly improved mechanical properties compared to their single component analogs. The HAMA-GelMA hydrogels exhibited remarkable tunability behavior and may be useful for cardiovascular tissue engineering applications.
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影响因子：
3.4
作者：
Camci-Unal G;Aubin H;Ahari AF;Bae H;Nichol JW;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
2.8
作者：
Cimetta, Elisa;Pizzato, Sara;Elvassore, Nicola
通讯作者：
Elvassore, Nicola
影响因子：
14
作者：
Kosmala, JD;Henthorn, DB;Brannon-Peppas, L
通讯作者：
Brannon-Peppas, L
影响因子：
14
作者：
Ji, Y;Ghosh, K;Rafailovich, MH
通讯作者：
Rafailovich, MH
影响因子：
--
作者：
Benton JA;DeForest CA;Vivekanandan V;Anseth KS
通讯作者：
Anseth KS