Surface-modified hyaluronic acid hydrogels to capture endothelial progenitor cells.
Surface-modified hyaluronic acid hydrogels to capture endothelial progenitor cells.
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DOI：
10.1039/c0sm00508h
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发表时间：
2010-10-21
期刊：
影响因子：
3.4
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Camci-Unal G;Aubin H;Ahari AF;Bae H;Nichol JW;Khademhosseini A
有效治疗受损心血管组织的一个主要挑战是促进受损组织和植入装置的内皮化。因此，需要新的促进血管内皮化的生物材料来加强血管修复。这项工作的目标是开发抗体修饰的多糖基水凝胶，可以选择性地捕获内皮祖细胞(EPC)。我们发现，CD34抗体固定在透明质酸(HA)水凝胶上提供了一个合适的表面来捕获内皮祖细胞。CD34抗体固定化对EPC黏附的影响与抗体浓度有关。内皮祖细胞在1%HA凝胶上的黏附率最高，为52.2个/mm~2，修饰后的凝胶中含有25μg/mL的−-1抗体。与内皮祖细胞相比，巨噬细胞在这些修饰的水凝胶表面没有表现出明显的附着，这表明了该系统的选择性。只含有HA的水凝胶，无论有没有固定化的CD34，都不允许内皮祖细胞在细胞接种后48小时扩散，即使细胞附着在水凝胶表面。为了促进内皮祖细胞的铺展，合成了2%(w/v)的含有HA水凝胶的明胶甲基丙烯酸酯(GelMA)，并证明了它能改善细胞的铺展和伸长。这一策略可能有助于提高人工心脏瓣膜等植入物的生物相容性，或在需要形成血管结构的其他组织工程应用中。
A major challenge to the effective treatment of injured cardiovascular tissues is the promotion of endothelialization of damaged tissues and implanted devices. For this reason, there is a need for new biomaterials that promote endothelialization to enhance vascular repair. The goal of this work was to develop antibody-modified polysaccharide-based hydrogels that could selectively capture endothelial progenitor cells (EPCs). We showed that CD34 antibody immobilization on hyaluronic acid (HA) hydrogels provides a suitable surface to capture EPCs. The effect of CD34 antibody immobilization on EPC adhesion was found to be dependent on antibody concentration. The highest level of EPC attachment was found to be 52.2 cells per mm2 on 1% HA gels modified with 25 μg mL−1 antibody concentration. Macrophages did not exhibit significant attachment on these modified hydrogel surfaces compared to the EPCs, demonstrating the selectivity of the system. Hydrogels containing only HA, with or without immobilized CD34, did not allow for spreading of EPCs 48 h after cell seeding, even though the cells were adhered to the hydrogel surface. To promote spreading of EPCs, 2% (w/v) gelatin methacrylate (GelMA) containing HA hydrogels were synthesized and shown to improve cell spreading and elongation. This strategy could potentially be useful to enhance the biocompatibility of implants such as artificial heart valves or in other tissue engineering applications where formation of vascular structures is required.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
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de Winter, Robbert J.
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作者：
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通讯作者：
Matsuda, T
影响因子：
14
作者：
Khademhosseini, A;Suh, KY;Langer, R
通讯作者：
Langer, R