Anatomical basis for cell transplantation into mouse seminiferous tubules.
Anatomical basis for cell transplantation into mouse seminiferous tubules.
复制标题
细胞移植到小鼠生精小管的解剖学基础。
作者：
U. Silvan;J. Aréchaga
细胞移植到生精小管中是研究影响睾丸的生理和病理条件的有用技术。然而，精确的三维组织，特别是生精网络的复杂连接性尚未得到彻底表征。迄今为止，解决这些问题的技术方法包括立体显微镜下的手动解剖、组织学连续切片的重建和对比染料的注射，但所有这些都只产生了部分信息。在这里，我们使用一种基于自聚合树脂显微注射，然后对周围软组织进行化学消化的方法，揭示了生精小管支架及其连接的细节。通过扫描电子显微镜研究了这些睾丸生精网络的复制品。目前的结果不仅为更精确地显微注射到小鼠生精小管中奠定了形态学基础，而且还能够更深入地研究睾丸的生理和胚胎学特征。
Cell transplantation into the seminiferous tubules is a useful technique for the study of physiological and pathological conditions affecting the testis. However, the precise three-dimensional organization and, particularly, the complex connectivity of the seminiferous network have not yet been thoroughly characterized. To date, the technical approaches to address these issues have included manual dissection under the stereomicroscope, reconstruction of histological serial sections, and injection of contrast dyes, but all of them have yielded only partial information. Here, using an approach based on the microinjection of a self-polymerizing resin followed by chemical digestion of the surrounding soft tissues, we reveal fine details of the seminiferous tubule scaffold and its connections. These replicas of the testis seminiferous network were studied by scanning electron microscopy. The present results not only establish a morphological basis for more precise microinjection into the mouse seminiferous tubules but also enable a more profound investigation of physiological and embryological features of the testis.
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影响因子：
3.6
作者：
Ogawa, T;Dobrinski, I;Brinster, RL
通讯作者：
Brinster, RL
影响因子：
3.6
作者：
Dobrinski, I;Avarbock, MR;Brinster, RL
通讯作者：
Brinster, RL
DOI：
10.1387/ijdb.9074943
发表时间：
2003-02
期刊：
The International journal of developmental biology
影响因子：
--
作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
通讯作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
DOI：
10.1073/pnas.91.24.11303
发表时间：
1994-11-22
影响因子：
11.1
作者：
BRINSTER, RL;AVARBOCK, MR
通讯作者：
AVARBOCK, MR