A temporal signature of epidermal growth factor signaling regulates the differentiation of germline cells in testes of Drosophila melanogaster.
A temporal signature of epidermal growth factor signaling regulates the differentiation of germline cells in testes of Drosophila melanogaster.
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DOI：
10.1371/journal.pone.0070678
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Schulz C
中科院分区：
文献类型：
--
作者：
Hudson AG;Parrott BB;Qian Y;Schulz C
干细胞的组织补充遵循一系列精确的事件，在此期间，干细胞子细胞首先通过有丝分裂运输放大分裂增殖，然后进入终末分化。在这里，我们讨论干细胞子体是如何被引导通过早期发展步骤的。在果蝇睾丸中，体细胞囊泡细胞包裹着增殖和分化的生殖系细胞，生殖系细胞和周围的囊泡细胞单元通常被称为囊泡。通过表征果蝇表皮生长因子(EGF)信号的减少或增加，我们表明EGF在包囊中根据其剂量触发不同的反应。除了先前报道的在包囊形成过程中需要EGF信号外，低剂量的EGF信号是生殖系细胞通过转运扩增分裂所必需的，而高剂量的EGF信号促进末端分化。在表达固有活性的EGF受体(EGFR)的睾丸和同时表达分泌型EGF和EGFR的囊性细胞中，终末分化被促进，但在仅表达EGF或仅表达EGFR的睾丸中不被促进。我们认为，随着包囊的发展，EGF信号的时间特征是通过胚系细胞产生活性配体和包囊细胞上可用的受体分子的数量的协调增加而产生的。
Tissue replenishment from stem cells follows a precise cascade of events, during which stem cell daughters first proliferate by mitotic transit amplifying divisions and then enter terminal differentiation. Here we address how stem cell daughters are guided through the early steps of development. In Drosophila testes, somatic cyst cells enclose the proliferating and differentiating germline cells and the units of germline and surrounding cyst cells are commonly referred to as cysts. By characterizing flies with reduced or increased Epidermal Growth Factor (EGF) signaling we show that EGF triggers different responses in the cysts dependent on its dose. In addition to the previously reported requirement for EGF signaling in cyst formation, a low dose of EGF signaling is required for the progression of the germline cells through transit amplifying divisions, and a high dose of EGF signaling promotes terminal differentiation. Terminal differentiation was promoted in testes expressing a constitutively active EGF Receptor (EGFR) and in testes expressing both a secreted EGF and the EGFR in the cyst cells, but not in testes expressing either only EGF or only EGFR. We propose that as the cysts develop, a temporal signature of EGF signaling is created by the coordinated increase of both the production of active ligands by the germline cells and the amount of available receptor molecules on the cyst cells.
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