Human liver cancer cells and endothelial cells incorporate iodised oil.
Human liver cancer cells and endothelial cells incorporate iodised oil.
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DOI：
10.1038/bjc.1996.156
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发表时间：
1996-04
影响因子：
8.8
通讯作者：
Hobbs, KEF
中科院分区：
文献类型：
--
作者：
Bhattacharya, S;Dhillon, AP;Winslet, MC;Davidson, BR;Shukla, N;Gupta, SD;AlMufti, R;Hobbs, KEF
通过肝动脉施用的碘化油（碘化油）可长期选择性地定位于原发性肝细胞癌（肝细胞癌或 HCC），并已被用作细胞毒剂的载体。尽管有临床使用，肿瘤中碘油滞留的机制仍不清楚，肿瘤脉管系统中油滴的栓塞是普遍的假设。我们研究了肿瘤和内皮细胞在碘油滞留中的作用。将培养的人肝肿瘤 (Hep G2) 细胞和人脐静脉内皮细胞暴露于碘油中。使用选择性银浸渍染色的光学显微镜和透射电子显微镜显示两种细胞类型都掺入了碘油，可能是通过胞饮作用。这与细胞裂解、细胞复制或放射性标记亮氨酸摄取方面的细胞损伤无关。对手术切除或肝移植时偶然发现的 24 个 HCC（事先动脉注射碘化油）进行的组织学分析显示，肿瘤细胞和肿瘤血管内皮细胞的细胞质中存在碘化油囊泡。因此，在体外和体内，碘油可能将细胞毒性剂直接递送到肿瘤细胞和内皮细胞中。这也可能适用于其他脂质和其他人类肿瘤。这些发现具有重要的治疗意义。
Iodised oil (lipiodol) administered via the hepatic artery localises selectively in primary liver cell cancers (hepatocellular carcinomas or HCCs) for prolonged periods and has been used as a vehicle for cytotoxic agents. Despite clinical use, the mechanism of lipiodol retention by tumours has remained unclear, embolisation of oil droplets in the tumour vasculature being the prevailing hypothesis. We have investigated the role of tumour and endothelial cells in lipiodol retention. Human liver tumour (Hep G2) cells and human umbilical vein endothelial cells in culture were exposed to lipiodol. Light microscopy using selective silver impregnation stains and transmission electron microscopy revealed lipiodol incorporation by both cell types, probably by pinocytosis. This was not associated with cellular injury in terms of cell lysis, cell replication or radio-labelled leucine uptake. Histological analysis of 24 HCCs either surgically resected or discovered incidentally at liver transplantation (with prior arterial injection of lipiodol) revealed vesicles of lipiodol in the cytoplasm of tumour cells and endothelial cells lining tumour vessels. Thus, lipiodol is likely to deliver cytotoxic agents directly into tumour cells and endothelial cells, both in vitro and in vivo. This may also apply to other lipids and to other human tumours. These findings have significant therapeutic implications.
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影响因子：
8.8
作者：
HUSSEY, HJ;TISDALE, MJ
通讯作者：
TISDALE, MJ
影响因子：
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影响因子：
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作者：
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通讯作者：
OHNISHI, K