Response of Astrocyte Subpopulations Following Spinal Cord Injury.
Response of Astrocyte Subpopulations Following Spinal Cord Injury.
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脊髓损伤后星形胶质细胞亚群的反应。
DOI：
10.3390/cells11040721
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发表时间：
2022-02-18
期刊：
影响因子：
6
通讯作者：
Lepore AC
中科院分区：
文献类型：
--
作者：
Allahyari RV;Heinsinger NM;Hwang D;Jaffe DA;Rasouli J;Shiers S;Thomas SJ;Price TJ;Rostami A;Lepore AC
越来越多的人认识到星形胶质细胞在中枢神经系统（CNS）区域之间和区域内的异质性，以及在完整状态和疾病状态之间的异质性。最近的工作确定了成熟大脑中的多个星形胶质细胞亚群。有趣的是，一个亚群（人口C）被证明具有显着增强的体外突触特性，与其他星形胶质细胞亚群的成年皮层和脊髓相比。脊髓损伤（SCI）后，受损的神经元失去与神经元伙伴的突触连接，导致持续的功能丧失。我们确定SCI是否通过向更大比例的C群细胞转移和/或增加一个或多个星形胶质细胞亚群内相关突触形成相关基因的表达来诱导增强的突触调节星形胶质细胞表型。使用流式细胞术和RNAscope原位杂交，我们发现，星形胶质细胞亚群分布在脊髓没有改变到一个选择性突触形成表型小鼠颈半切除型SCI。我们还发现，脊髓星形胶质细胞表达突触形成相关基因的程度相似的亚群，以及在未受伤和SCI条件之间的不变的方式。最后，我们证实了这些星形胶质细胞亚群也存在于人类脊髓中，分布与小鼠相似，这表明脊髓星形胶质细胞异质性在物种间可能是保守的。
There is growing appreciation for astrocyte heterogeneity both across and within central nervous system (CNS) regions, as well as between intact and diseased states. Recent work identified multiple astrocyte subpopulations in mature brain. Interestingly, one subpopulation (Population C) was shown to possess significantly enhanced synaptogenic properties in vitro, as compared with other astrocyte subpopulations of adult cortex and spinal cord. Following spinal cord injury (SCI), damaged neurons lose synaptic connections with neuronal partners, resulting in persistent functional loss. We determined whether SCI induces an enhanced synaptomodulatory astrocyte phenotype by shifting toward a greater proportion of Population C cells and/or increasing expression of relevant synapse formation-associated genes within one or more astrocyte subpopulations. Using flow cytometry and RNAscope in situ hybridization, we found that astrocyte subpopulation distribution in the spinal cord did not change to a selectively synaptogenic phenotype following mouse cervical hemisection-type SCI. We also found that spinal cord astrocytes expressed synapse formation-associated genes to a similar degree across subpopulations, as well as in an unchanged manner between uninjured and SCI conditions. Finally, we confirmed these astrocyte subpopulations are also present in the human spinal cord in a similar distribution as mouse, suggesting possible conservation of spinal cord astrocyte heterogeneity across species.
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影响因子：
6.1
作者：
Cheng L;Sami A;Ghosh B;Goudsward HJ;Smith GM;Wright MC;Li S;Lepore AC
通讯作者：
Lepore AC
影响因子：
25
作者：
John Lin CC;Yu K;Hatcher A;Huang TW;Lee HK;Carlson J;Weston MC;Chen F;Zhang Y;Zhu W;Mohila CA;Ahmed N;Patel AJ;Arenkiel BR;Noebels JL;Creighton CJ;Deneen B
通讯作者：
Deneen B
影响因子：
16.2
作者：
Burda JE;Sofroniew MV
通讯作者：
Sofroniew MV
影响因子：
6.2
作者：
Goulao, Miguel;Ghosh, Biswarup;Lepore, Angelo C.
通讯作者：
Lepore, Angelo C.
影响因子：
16.6
作者：
Batiuk, Mykhailo Y.;Martirosyan, Araks;Holt, Matthew G.
通讯作者：
Holt, Matthew G.