Defining stem and progenitor cells within adipose tissue.
Defining stem and progenitor cells within adipose tissue.
复制标题
DOI：
10.1089/scd.2008.0117
复制
发表时间：
2008-12
影响因子：
4
通讯作者：
Lin CS
中科院分区：
文献类型：
--
作者：
Lin G;Garcia M;Ning H;Banie L;Guo YL;Lue TF;Lin CS
脂肪组织来源的干细胞（ADSC）通常从均质化脂肪组织的基质血管部分（SVF）中分离。新鲜分离的ADSC显示不同于培养的ADSC的表面标志物，但两种细胞制剂都能够多潜能分化。最近的研究推断，这些祖细胞可能存在于血管周围位置，在那里它们似乎共表达CD 34和平滑肌肌动蛋白（α-SMA），但不表达CD 31。然而，这些研究只提供了有限的组织学证据来支持这种说法。在本研究中，我们采用免疫组化和免疫荧光更精确地确定ADSC在人体脂肪组织中的位置。我们的研究结果表明，α-SMA和CD 31定位在平滑肌和内皮细胞，分别在所有的血管检查。CD 34定位于内膜（内皮）和外膜，两者均不表达α-SMA。小生境标记Wnt 5a仅限于血管壁，在壁平滑肌细胞内。令人惊讶的是，广泛接受的间充质干细胞标记物STRO-1仅在毛细血管和小动脉的内皮中表达，而不在动脉的内皮中表达。胚胎干细胞标志物SSEA 1定位于毛细血管中的细胞周位置和小动脉的某些平滑肌细胞中。表达胚胎干细胞标记物端粒酶和OCT 4的细胞很少，仅在毛细血管中观察到。基于这些发现和现有文献中收集的证据，我们提出ADSC是处于不同分化阶段的血管前体（干）细胞。在其天然组织中，处于分化早期阶段的ADSC可以分化为组织特异性细胞，如脂肪细胞。分离的ADSC可以被诱导分化成其他细胞类型，如成骨细胞和软骨细胞。
Adipose tissue-derived stem cells (ADSC) are routinely isolated from the stromal vascular fraction (SVF) of homogenized adipose tissue. Freshly isolated ADSC display surface markers that differ from those of cultured ADSC, but both cell preparations are capable of multipotential differentiation. Recent studies have inferred that these progenitors may reside in a perivascular location where they appeared to co-express CD34 and smooth muscle actin (α–SMA) but not CD31. However, these studies provided only limited histological evidence to support such assertions. In the present study we employed immunohistochemistry and immunofluorescence to define more precisely the location of ADSC within human adipose tissue. Our results show that α–SMA and CD31 localized within smooth muscle and endothelial cells, respectively, in all blood vessels examined. CD34 localized to both the intima (endothelium) and adventitia, neither of which expressed α–SMA. The niche marker Wnt5a was confined exclusively to the vascular wall, within mural smooth muscle cells. Surprisingly, the widely accepted mesenchymal stem cell marker STRO-1 was expressed exclusively in the endothelium of capillaries and arterioles but not in the endothelium of arteries. The embryonic stem cell marker SSEA1 localized to a pericytic location in capillaries and in certain smooth muscle cells of arterioles. Cells expressing the embryonic stem cell markers telomerase and OCT4 were rare and observed only in capillaries. Based on these findings and evidence gathered from the existing literature, we propose that ADSC are vascular precursor (stem) cells at various stages of differentiation. In their native tissue, ADSC at early stages of differentiation can differentiate into tissue-specific cells such as adipocytes. Isolated, ADSC can be induced to differentiate into additional cell types such as osteoblasts and chondrocytes.
登录
查看更多内容
影响因子：
2.4
作者：
Housman, TS;Lawrence, N;Fleischer, AB
通讯作者：
Fleischer, AB
影响因子：
2.9
作者：
Ning, Hongxiu;Lin, Guiting;Lin, Ching-Shwun
通讯作者：
Lin, Ching-Shwun
影响因子：
37.8
作者：
Miranville, A;Heeschen, C;Bouloumié, A
通讯作者：
Bouloumié, A
影响因子：
20.1
作者：
Bouloumié, A;Drexler, HCA;Busse, R
通讯作者：
Busse, R
影响因子：
4
作者：
Izadpanah, Reza;Trygg, Cynthia;Bunnell, Bruce A.
通讯作者：
Bunnell, Bruce A.