Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy.
Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy.
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DOI：
10.1038/s41598-021-89715-1
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发表时间：
2021-05-13
影响因子：
4.6
通讯作者：
Churcher TS
中科院分区：
文献类型：
--
作者：
Da DF;McCabe R;Somé BM;Esperança PM;Sala KA;Blight J;Blagborough AM;Dowell F;Yerbanga SR;Lefèvre T;Mouline K;Dabiré RK;Churcher TS
迫切需要高通量、经济实惠的方法来检测昆虫媒介体内的病原体，以促进监测。近红外光谱 (NIRS) 已显示出在实验室中检测虫媒病毒和疟疾的前景，但尚未在现场条件下进行评估。在这里，我们研究了 NIRS 识别按蚊中恶性疟原虫的能力。对实验室饲养的感染野生疟疾寄生虫的蚊子进行训练的 NIRS 模型可以以中等准确度检测出类似蚊子中的寄生虫，但无法检测到自然感染的野外捕获的蚊子中的卵囊或子孢子。在野外蚊子上训练的模型无法预测其他野外蚊子的感染状态。将分析限制在非传染性和高传染性状态的蚊子确实改善了预测，表明寄生虫数量较多的蚊子的敏感性和特异性可能更好。感染的检测似乎仅限于同质的蚊子群体，这降低了 NIRS 在检测蚊子体内疟疾的效用。
There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise to detect arbovirus and malaria in the laboratory but has not been evaluated in field conditions. Here we investigate the ability of NIRS to identify Plasmodium falciparum in Anopheles coluzzii mosquitoes. NIRS models trained on laboratory-reared mosquitoes infected with wild malaria parasites can detect the parasite in comparable mosquitoes with moderate accuracy though fails to detect oocysts or sporozoites in naturally infected field caught mosquitoes. Models trained on field mosquitoes were unable to predict the infection status of other field mosquitoes. Restricting analyses to mosquitoes of uninfectious and highly-infectious status did improve predictions suggesting sensitivity and specificity may be better in mosquitoes with higher numbers of parasites. Detection of infection appears restricted to homogenous groups of mosquitoes diminishing NIRS utility for detecting malaria within mosquitoes.
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