Differential expression of progesterone receptor isoforms A and B in the normal ovary, and in benign, borderline, and malignant ovarian tumors.
Differential expression of progesterone receptor isoforms A and B in the normal ovary, and in benign, borderline, and malignant ovarian tumors.
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DOI：
10.1111/j.1349-7006.2002.tb01323.x
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发表时间：
2002-07
期刊：
影响因子：
--
通讯作者：
Sasano H
中科院分区：
文献类型：
--
作者：
Akahira J;Suzuki T;Ito K;Kaneko C;Darnel AD;Moriya T;Okamura K;Yaegashi N;Sasano H
众所周知，人类卵巢上皮性肿瘤与性类固醇相关，但黄体酮在这些肿瘤中的作用可能的生物学意义仍存在争议。在这项研究中，我们使用免疫组织化学和real - tune定量RT - PCR检测了两种黄体酮受体（PR）亚型PRA和PRB在正常卵巢组织和肿瘤卵巢组织以及来自正常卵巢表面上皮和卵巢上皮癌的细胞系中的差异表达模式，以进一步阐明黄体酮在卵巢肿瘤发展中的可能参与。正常卵巢组织（n=8）、良性卵巢组织（n=10）、交界性卵巢组织（n=8）和恶性卵巢组织（n=24）中PR亚型的中位H评分如下：PRA分别为194.0、171.0、49.5、0 (P<0.05)， PRB分别为175.0、180.5、251.5、168.5。在卵巢癌细胞系（OVCAR-3和Caov-3）中，17β -雌二醇增加了PRB/PRAB mRNA的比例，并且具有浓度依赖性和剂量依赖性。然而，在正常卵巢上皮细胞系（NOV-31）中添加17β -雌二醇后，这一比例没有改变。免疫印迹分析表明，PRB蛋白在OVCAR-3中的表达明显上调，而PRA和PRB亚型在11 - 31中均有所增加。这些结果表明，PRA的下调与卵巢上皮癌的发生有关。
Human epithelial ovarian neoplasm is well‐known to be sex steroid‐related, but the possible biological significance of progesterone actions in these tumors remains controversial. In this study, we examined the differential expression patterns of the two progesterone receptor (PR) isoforms, PRA and PRB, using immunohistochemistry and real‐tune quantitative RT‐PCR in normal and neoplastic ovarian tissues, and in cell lines derived from a normal ovarian surface epithelium and an ovarian epithelial carcinoma in order to further elucidate the possible involvement of progesterone in the development of ovarian neoplasms. The median H scores for PR isoforms in normal (n=8), benign (n=10), borderline (n=8) and malignant (n=24) ovarian tissues were as follows; PRA: 194.0, 171.0, 49.5, 0 (P<0.05), and PRB: 175.0, 180.5, 251.5, 168.5, respectively. In ovarian cancer cell lines (OVCAR–3 and Caov–3), the PRB/PRAB mRNA ratio was increased by 17β‐estradiol, both tune‐and dose‐dependently. However, this ratio was unaltered following the addition of 17β‐estradiol in a normal ovarian epithelial cell line (NOV–31). Immunoblotting analysis demonstrated that PRB protein expression was markedly up‐regulated in OVCAR–3, whereas the PRA and PRB isoforms both appeared to be increased in NOV–31. These results suggest that down‐regulation of PRA is associated with the development of ovarian epithelial carcinoma.
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影响因子：
11.4
作者：
KASTNER, P;KRUST, A;CHAMBON, P
通讯作者：
CHAMBON, P
影响因子：
3.7
作者：
LOUNIS, H;PROVENCHER, D;MESMASSON, AM
通讯作者：
MESMASSON, AM
影响因子：
45.3
作者：
AHLGREN, JD;ELLISON, NM;FRYER, JG
通讯作者：
FRYER, JG
DOI：
10.1111/j.1349-7006.2001.tb01095.x
发表时间：
2001-03
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Ariga N;Suzuki T;Moriya T;Kimura M;Inoue T;Ohuchi N;Sasano H
通讯作者：
Sasano H
影响因子：
56.9
作者：
EVANS, RM
通讯作者：
EVANS, RM