Pulmonary surfactant coating of multi-walled carbon nanotubes (MWCNTs) influences their oxidative and pro-inflammatory potential in vitro.
Pulmonary surfactant coating of multi-walled carbon nanotubes (MWCNTs) influences their oxidative and pro-inflammatory potential in vitro.
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DOI：
10.1186/1743-8977-9-17
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发表时间：
2012-05-24
影响因子：
10
通讯作者：
Rothen-Rutishauser B
中科院分区：
文献类型：
--
作者：
Gasser M;Wick P;Clift MJ;Blank F;Diener L;Yan B;Gehr P;Krug HF;Rothen-Rutishauser B
越来越多的关注已经表达了对潜在的不利健康影响，可能与人类接触吸入多壁碳纳米管（MWCNT）。因此，必须了解潜在的机制，并确定造成不利影响的关键因素。在肺泡中，多壁碳纳米管首先与肺表面活性剂相互作用。在该界面处，肺表面活性剂的蛋白质和脂质与多壁碳纳米管结合，影响其表面特性。本研究的目的是研究用肺表面活性剂预涂覆多壁碳纳米管是否对（i）人单核细胞衍生的巨噬细胞（MDM）单一培养物和（ii）人上皮气道屏障的复杂体外模型的潜在不良反应有影响。两种体外系统均暴露于预先涂有猪肺表面活性剂（Curosurf）或未涂有的MWCNT。多壁碳纳米管表面电荷的影响也进行了研究，在氨基（-NH 2）和羧基（-COOH）表面改性。多壁碳纳米管预涂固尔苏通过增加活性氧水平和减少MDM中细胞内谷胱甘肽消耗以及减少肿瘤坏死因子α（TNF-α）的释放来影响其氧化电位。此外，在暴露于Curosurf预涂覆的多壁碳纳米管后观察到细胞凋亡的诱导。在三细胞共培养物中，暴露于Curosurf预涂覆的MWCNTs后，白细胞介素-8（IL-8）的释放增加。在MDM和三细胞共培养物中，MWCNT官能化的影响较小。本研究清楚地表明，多壁碳纳米管与肺表面活性剂的预涂层超过管的功能化是决定其引起氧化应激，细胞因子/趋化因子释放和细胞凋亡的能力的关键因素。因此，在未来的肺体外风险评估研究中，应考虑用肺表面活性剂涂覆纳米物体。
Increasing concern has been expressed regarding the potential adverse health effects that may be associated with human exposure to inhaled multi-walled carbon nanotubes (MWCNTs). Thus it is imperative that an understanding as to the underlying mechanisms and the identification of the key factors involved in adverse effects are gained. In the alveoli, MWCNTs first interact with the pulmonary surfactant. At this interface, proteins and lipids of the pulmonary surfactant bind to MWCNTs, affecting their surface characteristics. Aim of the present study was to investigate if the pre-coating of MWCNTs with pulmonary surfactant has an influence on potential adverse effects, upon both (i) human monocyte derived macrophages (MDM) monocultures, and (ii) a sophisticated in vitro model of the human epithelial airway barrier. Both in vitro systems were exposed to MWCNTs either pre-coated with a porcine pulmonary surfactant (Curosurf) or not. The effect of MWCNTs surface charge was also investigated in terms of amino (−NH2) and carboxyl (−COOH) surface modifications. Pre-coating of MWCNTs with Curosurf affects their oxidative potential by increasing the reactive oxygen species levels and decreasing intracellular glutathione depletion in MDM as well as decreases the release of Tumour necrosis factor alpha (TNF-α). In addition, an induction of apoptosis was observed after exposure to Curosurf pre-coated MWCNTs. In triple cell-co cultures the release of Interleukin-8 (IL-8) was increased after exposure to Curosurf pre-coated MWCNTs. Effects of the MWCNTs functionalizations were minor in both MDM and triple cell co-cultures. The present study clearly indicates that the pre-coating of MWCNTs with pulmonary surfactant more than the functionalization of the tubes is a key factor in determining their ability to cause oxidative stress, cytokine/chemokine release and apoptosis. Thus the coating of nano-objects with pulmonary surfactant should be considered for future lung in vitro risk assessment studies.
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影响因子：
3.2
作者：
Chin, Shook-Fong;Baughman, Ray H.;Pantano, Paul
通讯作者：
Pantano, Paul
影响因子：
10.9
作者：
Brown, D. M.;Kinloch, I. A.;Stone, V.
通讯作者：
Stone, V.
影响因子：
6.2
作者：
Clift, Martin J. D.;Foster, E. Johan;Weder, Christoph
通讯作者：
Weder, Christoph
影响因子：
10.8
作者：
Carrero-Sanchez, J. C.;Elias, A. L.;Terrones, M.
通讯作者：
Terrones, M.
大鼠肺中灌输碳纳米管的生物分布和清除。
影响因子：
10
作者：
Elgrabli D;Floriani M;Abella-Gallart S;Meunier L;Gamez C;Delalain P;Rogerieux F;Boczkowski J;Lacroix G
通讯作者：
Lacroix G