Gold nanoparticles alleviates the lipopolysaccharide-induced intestinal epithelial barrier dysfunction.
Gold nanoparticles alleviates the lipopolysaccharide-induced intestinal epithelial barrier dysfunction.
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金纳米颗粒可缓解脂多糖引起的肠上皮屏障功能障碍。
DOI：
10.1080/21655979.2021.1972782
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发表时间：
2021-12
期刊：
影响因子：
4.9
通讯作者：
Lu X
中科院分区：
文献类型：
--
作者：
Wang Z;Cao Y;Zhang K;Guo Z;Liu Y;Zhou P;Liu Z;Lu X
纳米技术用于免疫反应操作以治疗各种人类疾病。在本研究中，我们探讨了Au纳米颗粒（AuNPs）对脂多糖（LPS）诱导的结肠上皮细胞NCM 460上皮屏障功能障碍和炎症反应的影响。细胞计数kit-8和流式细胞仪分析结果显示，LPS处理后，NCM 460细胞活力受到抑制，凋亡增加，而金纳米颗粒以剂量依赖的方式逆转了这些变化。通过检测异硫氰酸荧光素-葡聚糖的通量和跨上皮电阻来评价通透性。LPS可增加NCM 460细胞的通透性，促进屏障功能障碍。酶联免疫吸附试验结果显示，促炎因子和一氧化氮的浓度升高，LPS处理和降低的金纳米粒子。LPS加重了炎症反应，这是由金纳米颗粒拯救。此外，LPS促进核因子κ B和细胞外信号调节激酶/c-Jun NH-末端激酶信号通路的激活，这被AuNPs抑制。
Nanotechnology is used in the immune response manipulation to treat various human diseases. In the present study, we explored the effects of Au nanoparticles (AuNPs) on the lipopolysaccharide (LPS)-induced epithelial barrier dysfunction and inflammatory response of colonic epithelial NCM460 cells. According to the results of cell counting kit-8 and flow cytometry analysis, the viability of NCM460 cells was inhibited, and the apoptosis was increased after LPS treatment, and AuNPs reversed these changes in a dose-dependent way. The permeability was evaluated by detecting the flux of fluorescein isothiocyanate-dextran and transepithelial electrical resistance. LPS enhanced the permeability and promoted barrier dysfunction of NCM460 cells. Enzyme-linked immunosorbent sorbent assay results revealed that the concentrations of pro-inflammatory factors and nitric oxide were elevated by LPS treatment and decreased by the AuNPs. LPS aggravated the inflammatory response, which was rescued by the AuNPs. Moreover, LPS promoted the activation of the nuclear factor kappa-B and extracellular signal-regulated kinase/c-Jun NH-terminal kinase signaling pathways, which were inhibited by AuNPs.
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