Diversity of Light Sensing Molecules and Their Expression During the Embryogenesis of the Cuttlefish (Sepia officinalis).
Diversity of Light Sensing Molecules and Their Expression During the Embryogenesis of the Cuttlefish (Sepia officinalis).
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DOI：
10.3389/fphys.2020.521989
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发表时间：
2020
影响因子：
4
通讯作者：
Bonnaud-Ponticelli L
中科院分区：
文献类型：
--
作者：
Bonadè M;Ogura A;Corre E;Bassaglia Y;Bonnaud-Ponticelli L
眼睛的形态可能不同，但这些差异并不反映在分子水平上。事实上，感知光线的能力被认为来自相同的保守基因家族：视蛋白和隐花色素。尽管乌贼（Cetrapoda）以其视觉引导行为而闻名，但缺乏关于基因组中代表的不同视蛋白和隐花色素直向同源物及其表达的数据。在这里，我们研究了视蛋白，隐色素，但也视觉arrestins在软体动物的头足类动物的重点进化历史。我们确定了6个视蛋白，2隐花色素和1个视觉抑制蛋白在乌贼和我们表明这些家庭经历了几个重复的事件在软体动物：一个重复抑制蛋白家族和两个视蛋白家族。在乌贼，我们研究了这些基因的时间表达的胚胎从第23阶段到孵化的眼睛和他们的表达在两个眼外组织，皮肤和中枢神经系统（CNS =大脑+视叶）。我们在胚胎中发现，这些基因中的一些（Sof_reti-6，Sof_reti-1，Sof_reti-2，Sof_r-opsin 1和Sof_v-opsin 1）在眼睛中表达，而不在皮肤或CNS中表达。通过观察一只青少年和一只成年S。然而，从药用植物的基因组中可以看出，这些基因中的一些（Sof_r-opsin 1和Sof_reti1）似乎用于这些眼外组织中的光检测，但它们在发育中比在眼睛中更晚建立。我们还表明，它们的表达（除了Sof_106）经历了从阶段25到28的眼睛增加，从而证实了它们在乌贼胚胎通过卵囊感知光的能力中的作用。这项研究提出了一个问题，在乌贼胚胎的眼睛发育中的Sof_106的作用和xenopsin和r-opsin 2的作用和定位。因此，参与眼睛和眼外组织中的光检测的分子演员的多样性高于先前已知的。这些结果为研究新的分子，如信号转导级联的分子开辟了道路。
Eyes morphologies may differ but those differences are not reflected at the molecular level. Indeed, the ability to perceive light is thought to come from the same conserved gene families: opsins and cryptochromes. Even though cuttlefish (Cephalopoda) are known for their visually guided behaviors, there is a lack of data about the different opsins and cryptochromes orthologs represented in the genome and their expressions. Here we studied the evolutionary history of opsins, cryptochromes but also visual arrestins in molluscs with an emphasis on cephalopods. We identified 6 opsins, 2 cryptochromes and 1 visual arrestin in Sepia officinalis and we showed these families undergo several duplication events in Mollusca: one duplication in the arrestin family and two in the opsin family. In cuttlefish, we studied the temporal expression of these genes in the eyes of embryos from stage 23 to hatching and their expression in two extraocular tissues, skin and central nervous system (CNS = brain + optic lobes). We showed in embryos that some of these genes (Sof_CRY6, Sof_reti-1, Sof_reti-2, Sof_r-opsin1 and Sof_v-arr) are expressed in the eyes and not in the skin or CNS. By looking at a juvenile and an adult S. officinalis, it seems that some of these genes (Sof_r-opsin1 and Sof_reti1) are used for light detection in these extraocular tissues but that they set-up later in development than in the eyes. We also showed that their expression (except for Sof_CRY6) undergoes an increase in the eyes from stage 25 to 28 thus confirming their role in the ability of the cuttlefish embryos to perceive light through the egg capsule. This study raises the question of the role of Sof_CRY6 in the developing eyes in cuttlefish embryos and the role and localization of xenopsins and r-opsin2. Consequently, the diversity of molecular actors involved in light detection both in the eyes and extraocular tissues is higher than previously known. These results open the way for studying new molecules such as those of the signal transduction cascade.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
2.8
作者：
Kingston, Alexandra C. N.;Kuzirian, Alan M.;Cronin, Thomas W.
通讯作者：
Cronin, Thomas W.
影响因子：
4.8
作者：
GUREVICH, VV;DION, SB;BENOVIC, JL
通讯作者：
BENOVIC, JL
DOI：
10.1098/rspb.1961.0015
发表时间：
1961-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BOYCOTT, BB
通讯作者：
BOYCOTT, BB
影响因子：
64.5
作者：
Liscovitch-Brauer N;Alon S;Porath HT;Elstein B;Unger R;Ziv T;Admon A;Levanon EY;Rosenthal JJC;Eisenberg E
通讯作者：
Eisenberg E