Behavioral alterations in response to fear-provoking stimuli and tranylcypromine induced by perinatal exposure to bisphenol A and nonylphenol in male rats.
Behavioral alterations in response to fear-provoking stimuli and tranylcypromine induced by perinatal exposure to bisphenol A and nonylphenol in male rats.
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DOI：
10.1289/ehp.6961
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发表时间：
2004-08
影响因子：
10.4
通讯作者：
Yoshikawa Y
中科院分区：
文献类型：
--
作者：
Negishi T;Kawasaki K;Suzaki S;Maeda H;Ishii Y;Kyuwa S;Kuroda Y;Yoshikawa Y
本研究的目的是检查围产期暴露于两种主要的环境内分泌干扰化学品双酚A（BPA; 0.1 mg/kg/天口服）和壬基酚[NP; 0.1 mg/kg/天（低剂量）和10 mg/kg/天（高剂量）口服]，从妊娠第3天至产后第20天每日一次（经胎盘和哺乳期暴露）将导致F344大鼠雄性后代的行为改变。BPA和NP暴露均不影响旷场试验（8周龄）、自发运动活动测量（12周龄）或高架十字迷宫试验（14周龄）中的行为特征。被动回避测试（13周龄）表明，BPA和NP处理的后代往往延迟进入黑暗的隔间。在15周龄的主动回避测试显示，BPA处理的后代表现出显着较少的回避反应和低剂量NP处理的后代表现出略少的回避反应。此外，BPA处理的后代显着增加了失败的数量，以避免5秒的电击介绍与对照后代的电无条件刺激。在单胺破坏试验中，使用5 mg/kg（腹膜内）反苯环丙胺（Tcy），单胺氧化酶抑制剂，BPA处理和低剂量NP处理的后代在22-24周龄未能表现出显着的运动响应Tcy的增加，而对照组和高剂量NP处理的后代显着增加Tcy注射后的运动行为。此外，在单胺破坏试验中仅注射生理盐水时，低剂量NP处理的后代与对照后代相比表现出频繁的饲养。目前的结果表明，围产期低剂量BPA或NP暴露不可逆地影响了对引起恐惧的刺激的接受（例如，电击）以及单胺能神经通路。
The purpose of this study was to examine whether perinatal exposure to two major environmental endocrine-disrupting chemicals, bisphenol A (BPA; 0.1 mg/kg/day orally) and nonylphenol [NP; 0.1 mg/kg/day (low dose) and 10 mg/kg/day (high dose) orally] daily from gestational day 3 to postnatal day 20 (transplacental and lactational exposures) would lead to behavioral alterations in the male offspring of F344 rats. Neither BPA nor NP exposure affected behavioral characteristics in an open-field test (8 weeks of age), in a measurement of spontaneous motor activity (12 weeks of age), or in an elevated plus-maze test (14 weeks of age). A passive avoidance test (13 weeks of age) showed that both BPA- and NP-treated offspring tended to delay entry into a dark compartment. An active avoidance test at 15 weeks of age revealed that BPA-treated offspring showed significantly fewer avoidance responses and low-dose NP-treated offspring exhibited slightly fewer avoidance responses. Furthermore, BPA-treated offspring significantly increased the number of failures to avoid electrical unconditioned stimuli within 5-sec electrical shock presentation compared with the control offspring. In a monoamine-disruption test using 5 mg/kg (intraperitoneal) tranylcypromine (Tcy), a monoamine oxidase inhibitor, both BPA-treated and low-dose NP-treated offspring at 22–24 weeks of age failed to show a significant increment in locomotion in response to Tcy, whereas control and high-dose NP-treated offspring significantly increased locomotion behavior after Tcy injection. In addition, when only saline was injected during a monoamine-disruption test, low-dose NP-treated offspring showed frequent rearing compared with the control offspring. The present results indicate that perinatal low-dose BPA or NP exposure irreversibly influenced the reception of fear-provoking stimuli (e.g., electrical shock), as well as monoaminergic neural pathways.
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