Thickness-Sensing Sandwiched Plasmonic Biosensors Enable Label-Free Naked-Eye Antibody Quantification.
Thickness-Sensing Sandwiched Plasmonic Biosensors Enable Label-Free Naked-Eye Antibody Quantification.
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DOI：
10.1021/acs.nanolett.2c03732
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发表时间：
2022-12-14
期刊：
影响因子：
10.8
通讯作者：
Yang, Bai
中科院分区：
文献类型：
--
作者：
Nan, Jingjie;Sun, Weihong;Liu, Xin;Che, Yuanyuan;Shan, Hongli;Yue, Ying;Liu, Jiaxin;Wang, Lei;Liu, Kun;Xu, Wei;Zhang, Wenyan;Zhang, Songling;Liu, Bin;Hettie, Kenneth S.;Zhu, Shoujun;Zhang, Junhu;Yang, Bai
用于检测病毒抗体的临床血清学检测非常耗时，灵敏度/选择性较低，或者依赖复杂的检测仪器。在这里，我们开发了一种夹层等离子体生物传感器（SPB），通过利用夹层等离子体纳米结构中的距离依赖性电磁耦合来实现超灵敏厚度传感。 SPB 将纳米级范围内的厚度变化（灵敏度：~2% nm−1）定量放大为宏观可见信号，从而能够快速、无标记、肉眼检测目标生物分子种类（通过免疫结合事件引起的厚度变化）。作为概念验证，该测定提供了广泛的动态范围（7 个数量级）和低 LOD（约 0.3 pM），可实现极其准确的 SARS-CoV-2 抗体定量（灵敏度/特异性：100%/约 99%，使用便携式光纤设备）。该策略适用于护理点的高通量多重检测和基于智能手机的传感，可以扩展到病毒感染和疫苗接种领域之外的各种传感应用。
Clinical serology assays for detecting the antibodies of the virus are time-consuming, are less sensitive/selective, or rely on sophisticated detection instruments. Here, we develop a sandwiched plasmonic biosensor (SPB) for supersensitive thickness-sensing via utilizing the distance-dependent electromagnetic coupling in sandwiched plasmonic nanostructures. SPBs quantitatively amplify the thickness changes on the nanoscale range (sensitivity: ~2% nm−1) into macroscopically visible signals, thereby enabling the rapid, label-free, and naked-eye detection of targeted biomolecular species (via the thickness change caused by immunobinding events). As a proof of concept, this assay affords a broad dynamic range (7 orders of magnitude) and a low LOD (~0.3 pM), allowing for the extremely accurate SARS-CoV-2 antibody quantification (sensitivity/specificity: 100%/~99%, with a portable optical fiber device). This strategy is suitable for high-throughput multiplexed detection and smartphone-based sensing at the point-of-care, which can be expanded for various sensing applications beyond the fields of viral infections and vaccination.
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影响因子：
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作者：
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