The minipig as an animal model to study Mycobacterium tuberculosis infection and natural transmission.
The minipig as an animal model to study Mycobacterium tuberculosis infection and natural transmission.
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DOI：
10.1016/j.tube.2017.07.003
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发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
Gonzalez-Juarrero M
中科院分区：
文献类型：
--
作者：
Ramos L;Obregon-Henao A;Henao-Tamayo M;Bowen R;Lunney JK;Gonzalez-Juarrero M
在流行国家，超过 20% 的结核病 (TB) 病例发生在婴儿和儿童中。目前研究成年期结核病的动物模型，但婴儿结核病的动物模型很少。在这里，我们建议使用小型猪作为动物模型来研究成人、青少年和婴儿结核病，包括自然传播。在这些研究中，两个月大的小型猪（代表人类的婴儿年龄）和六个月大的小型猪（代表人类的青春期）通过气溶胶途径感染高毒力临床菌株W-北京结核分枝杆菌（Mtb）HN878，并分别在攻击后监测11周或36周。在同一项研究中，受感染的动物和未受到攻击的动物被安置在一起。从感染者及其最初未受挑战的自然接触者的肺和胸淋巴结中回收了活细菌。细菌载量、肉眼病变和组织病理学显示与人类结核病中观察到的疾病谱相似。该研究未达到最终终点，因此无法注释活动性结核病的明确临床症状。结果表明，小型猪是 Mtb HN878 的实验宿主，其肺部的病理学结果与人类结核病中描述的病理学结果相似。重要的是，在感染结核杆菌的小型猪群内，会发生自然传播。
In endemic countries more than 20% of tuberculosis (TB) cases are in infants and children. Current animal models study TB during adulthood but animal models for infant TB are scarce. Here we propose that minipigs can be used as an animal model to study adult, adolescent and infant TB including natural transmission. In these studies, two-month old minipigs (representing infant age in humans) and six-month old minipigs (representing adolescence in humans) were infected via the aerosol route with hyper-virulent clinical strain W-Beijing Mycobacterium tuberculosis (Mtb) HN878 and were monitored for 11 or 36 weeks post-challenge, respectively. In the same studies, infected and unchallenged animals were housed together. Viable bacteria were recovered from pulmonary and thoracic lymph nodes from both - infected and their initially unchallenged natural contacts. Bacillary load, gross lesions and histopathology revealed similarities to the spectrum of disease observed in human TB. The study did not reach terminal end point, thus it was not possible to annotate definitive clinical symptoms of active TB. The results demonstrated that minipigs are experimental hosts of Mtb HN878, and the pathology developed in their lungs resembles pathological findings described in human TB. Importantly, within communities of Mtb infected minipigs natural transmission occurs.
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DOI：
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