Immobilization of amphiphilic polycations by catechol functionality for antimicrobial coatings.
Immobilization of amphiphilic polycations by catechol functionality for antimicrobial coatings.
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抗微生物涂层的儿茶酚功能将两亲和固定的固定。
DOI：
10.1021/la1046904
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发表时间：
2011-04-05
期刊：
影响因子：
--
通讯作者：
Kuroda K
中科院分区：
文献类型：
--
作者：
Han H;Wu J;Avery CW;Mizutani M;Jiang X;Kamigaito M;Chen Z;Xi C;Kuroda K
据报道，一种通过简单的浸涂程序制备抗菌表面的新策略。采用自由基聚合法合成了含十二烷基季铵、甲氧基乙基和邻苯二酚基团的单体不同摩尔比的两亲性聚阳离子。通过将载玻片浸入聚合物溶液中并随后干燥和加热来制备聚合物涂层。季铵侧链赋予涂层强大的抗菌活性，而甲氧基乙基侧链能够调节疏水/亲水平衡，儿茶酚基团促进聚合物固定到薄膜中。聚合物涂层表面在动态接触测定中显示出针对大肠杆菌和金黄色葡萄球菌的杀菌活性，并可在长达 96 小时内防止活大肠杆菌、金黄色葡萄球菌和鲍曼不动杆菌的积累。涂层表面的原子力显微镜 (AFM) 图像表明，除最疏水性的聚合物外，所有聚合物的表面几乎表现出相同的光滑度。不含甲氧基乙基侧链的疏水性聚合物显示出清晰的聚合物域结构，导致高表面粗糙度。表面结构的和频发生（SFG）振动光谱表征表明，十二烷基链主要位于涂层的表面-空气界面。 SFG 还表明儿茶酚的苯基在基材表面上定向。这些结果支持了我们的假设，即儿茶酚基团的粘合或交联功能阻止了聚合物的浸出，从而允许通过将亲水性组分掺入聚合物链中来调节两亲平衡以获得有效的杀菌活性。
A new strategy to prepare antimicrobial surfaces by a simple dip-coating procedure is reported. Amphiphilic polycations with different mole ratios of monomers containing dodecyl quaternary ammonium, methoxyethyl, and catechol groups were synthesized by free-radical polymerization. The polymer coatings were prepared by immersing glass slides into a polymer solution and subsequent drying and heating. The quaternary ammonium side chains endow the coatings with potent antibacterial activity, while the methoxyetyhyl side chains enable tuning the hydrophobic/hydrophilic balance and the catachol groups promote immobilization of the polymers into films. The polymer coated surfaces displayed bactericidal activity against Escherichia coli and Staphylococcus aureus in a dynamic contact assay and prevented accumulation of viable E. coli, S. aureus, and Acinetobacter baumannii for up to 96 hours. Atomic force microscopy (AFM) images of coating surfaces indicated that the surfaces exhibit virtually the same smoothness for all polymers except the most hydrophobic. The hydrophobic polymer without methoxyethyl side chains showed clear structuring into polymer domains, causing high surface roughness. Sum-frequency generation (SFG) vibrational spectroscopy characterization of the surface structures demonstrated that the dodecyl chains are predominantly localized at the surface-air interface of the coatings. SFG also showed that the phenyl groups of the catechols are oriented on the substrate surface. These results support our hypothesis that the adhesive or cross-linking functionality of catechol groups discourages leaching of polymers, allowing tuning of the amphiphilic balance by incorporating hydrophilic components into the polymer chains to gain potent biocidal activity.
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影响因子：
2.9
作者：
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通讯作者：
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4.2
作者：
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作者：
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15
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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