Pomegranate (Punica granatum) Phenolics Ameliorate Hydrogen Peroxide-Induced Oxidative Stress and Cytotoxicity in Human Keratinocytes.
Pomegranate (Punica granatum) Phenolics Ameliorate Hydrogen Peroxide-Induced Oxidative Stress and Cytotoxicity in Human Keratinocytes.
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DOI：
10.1016/j.jff.2019.02.015
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发表时间：
2019-03
影响因子：
5.6
通讯作者：
Ma H
中科院分区：
文献类型：
--
作者：
Liu C;Guo H;DaSilva NA;Li D;Zhang K;Wan Y;Gao XH;Chen HD;Seeram NP;Ma H
石榴酚已被报道对皮肤有益，但其作用机制尚不清楚。在此，我们研究了一种标准化的商业石榴提取物(PE；Pomella®)及其酚类化合物，包括番泻素(PA)、鞣花酸(EA)和尿素A(UA)，以研究它们对过氧化氢(H_2O_2)诱导的人角质形成细胞HaCaT细胞氧化应激和细胞毒性的保护作用。PE、PA和EA可使H_2O_2诱导的HaCaT细胞产生的ROS分别减少1.03、1.37和2.67倍。PE、PA和UA使H_2O_2刺激的HaCaT细胞存活率分别提高89.9%、94.9%和90.0%。PE、PA和UA使细胞凋亡率分别减少3.39%、7.11%和8.26%。PE、PA和UA使过氧化氢刺激的caspase-3水平分别降低2.31倍、2.06倍和2.68倍。这种PE及其酚类化合物对过氧化氢诱导的角质形成细胞的氧化应激和细胞毒性的改善作用支持了它们作为天然化妆品用于皮肤健康。
Pomegranate phenolics have been reported to exert skin beneficial effects but their mechanisms of action remain unclear. Herein, we investigated a standardized commercial pomegranate extract (PE; Pomella®) and its phenolics including punicalagin (PA), ellagic acid (EA), and urolithin A (UA) for their protective effects against hydrogen peroxide (H2O2)-induced oxidative stress and cytotoxicity in human keratinocyte HaCaT cells. PE, PA, and EA reduced the production of H2O2-induced ROS in HaCaT cells by 1.03-, 1.37-, and 2.67-fold, respectively. PE, PA, and UA increased the viability of H2O2-stimulated HaCaT cells by 89.9, 94.9, and 90.0%, respectively. PE, PA, and UA reduced apoptotic cell populations by 3.39, 7.11, and 8.26%, respectively. In addition, PE, PA and UA decreased H2O2-stimulated caspase-3 level by 2.31-, 2.06-, and 2.68-fold, respectively. The ameliorative effects of this PE and its phenolics against the H2O2-induced oxidative stress and cytotoxicity in keratinocytes support their utilization as natural cosmeceuticals for skin health.
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影响因子：
4.3
作者：
Qiu, Zhenpeng;Zhou, Benhong;Zhu, Fan
通讯作者：
Zhu, Fan
影响因子：
6.1
作者：
Pacheco-Palencia, Lisbeth A.;Noratto, Giuliana;Mertens-Talcott, Susanne U.
通讯作者：
Mertens-Talcott, Susanne U.
影响因子：
4.4
作者：
Ma H;DaSilva NA;Liu W;Nahar PP;Wei Z;Liu Y;Pham PT;Crews R;Vattem DA;Slitt AL;Shaikh ZA;Seeram NP
通讯作者：
Seeram NP
DOI：
10.4161/derm.22028
发表时间：
2012-07-01
期刊：
Dermato-endocrinology
影响因子：
--
作者：
Gkogkolou P;Böhm M
通讯作者：
Böhm M
影响因子：
6.1
作者：
Gonzalez-Sarrias, Antonio;Angeles Nunez-Sanchez, Maria;Carlos Espin, Juan
通讯作者：
Carlos Espin, Juan