3D printing of drug-eluting bioactive multifunctional coatings for orthopedic applications.
3D printing of drug-eluting bioactive multifunctional coatings for orthopedic applications.
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DOI：
10.18063/ijb.v9i2.661
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发表时间：
2023
影响因子：
8.4
通讯作者：
Desai, Salil
中科院分区：
文献类型：
--
作者：
Adarkwa, Eben;Roy, Abhijit;Ohodnicki, John;Lee, Boeun;Kumta, Prashant N.;Desai, Salil
关键词：
采用多层生物功能聚合物涂层对钛合金基体进行表面改性，实现了三维打印。聚乳酸-羟基乙酸（PLGA）和聚己内酯（PCL）聚合物分别包埋无定形磷酸钙（ACP）和万古霉素（VA）治疗剂，促进骨整合和抗菌活性。与PLGA涂层相比，PCL涂层显示出负载acp配方的均匀沉积模式，并且增强了细胞在钛合金基体上的附着力。扫描电子显微镜和傅里叶变换红外光谱证实了ACP颗粒的纳米复合结构，显示出与聚合物的强结合。细胞活力数据显示，MC3T3成骨细胞在聚合物涂层上的增殖与阳性对照相当。体外活/死评估表明，与PCL涂层的20层（稳定释放）相比，10层（ACP爆发释放）的细胞附着力更高。负载抗菌药物VA的PCL涂层显示出可调节的释放动力学特征，这取决于涂层的多层设计和药物含量。此外，涂层释放的活性VA浓度高于最低抑菌浓度和最低杀菌浓度，表明其对金黄色葡萄球菌菌株具有抑制作用。本研究为开发抗菌生物相容性涂层促进骨科种植体骨整合提供了基础。
Three-dimensional (3D) printing is implemented for surface modification of titanium alloy substrates with multilayered biofunctional polymeric coatings. Poly(lactic-co-glycolic) acid (PLGA) and polycaprolactone (PCL) polymers were embedded with amorphous calcium phosphate (ACP) and vancomycin (VA) therapeutic agents to promote osseointegration and antibacterial activity, respectively. PCL coatings revealed a uniform deposition pattern of the ACP-laden formulation and enhanced cell adhesion on the titanium alloy substrates as compared to the PLGA coatings. Scanning electron microscopy and Fourier-transform infrared spectroscopy confirmed a nanocomposite structure of ACP particles showing strong binding with the polymers. Cell viability data showed comparable MC3T3 osteoblast proliferation on polymeric coatings as equivalent to positive controls. In vitro live/dead assessment indicated higher cell attachments for 10 layers (burst release of ACP) as compared to 20 layers (steady release) for PCL coatings. The PCL coatings loaded with the antibacterial drug VA displayed a tunable release kinetics profile based on the multilayered design and drug content of the coatings. Moreover, the concentration of active VA released from the coatings was above the minimum inhibitory concentration and minimum bactericidal concentration, demonstrating its effectiveness against Staphylococcus aureus bacterial strain. This research provides a basis for developing antibacterial biocompatible coatings to promote osseointegration of orthopedic implants.
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影响因子：
5
作者：
Aldawood FK;Andar A;Desai S
通讯作者：
Desai S
DOI：
10.3390/ma14102548
发表时间：
2021-05-14
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Gaikwad A;Desai S
通讯作者：
Desai S
影响因子：
3.7
作者：
Neut D;Kluin OS;Crielaard BJ;van der Mei HC;Busscher HJ;Grijpma DW
通讯作者：
Grijpma DW
影响因子：
4.2
作者：
Antoci, Valentin, Jr.;Adams, Christopher S.;Parvizi, Javad
通讯作者：
Parvizi, Javad
影响因子：
2.7
作者：
Fuller, SB;Wilhelm, EJ;Jacobson, JM
通讯作者：
Jacobson, JM