Functional impairment of PRRSV-specific peripheral CD3+CD8high cells.
Functional impairment of PRRSV-specific peripheral CD3+CD8high cells.
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DOI：
10.1051/vetres/2009029
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发表时间：
2009-09
影响因子：
4.4
通讯作者：
Nauwynck HJ
中科院分区：
文献类型：
--
作者：
Costers S;Lefebvre DJ;Goddeeris B;Delputte PL;Nauwynck HJ
猪繁殖与呼吸综合征病毒（PRRSV）在感染猪的肺和淋巴组织中的复制在中和抗体出现之前已经强烈减少，这表明在这些部位消除PRRSV涉及其他免疫机制。本研究旨在确定PRRSV莱利斯塔病毒（LV）特异性细胞毒性T淋巴细胞（CTL）是否能有效清除PRRSV感染的肺泡巨噬细胞。因此，用PRRSV感染的肺泡巨噬细胞作为靶细胞和来自PRRSV感染的猪的自体外周血单核细胞（PBMC）作为PRRSV特异性CTL的来源进行CTL测定。将3头PRRSV感染猪的PBMC直接用于CTL测定，或在体外再刺激后使用。用伪狂犬病病毒（PRV）海棠感染的肺泡巨噬细胞和来自2头PRV海棠接种猪的自体PBMC进行CTL测定以验证测定。在源自PRRSV感染的猪的新鲜分离的PBMC中，直到实验结束（感染后56天- dpi）才检测到针对PRRSV感染的巨噬细胞的CTL活性。在体外用PRRSV再刺激PBMC导致从14 dpi开始的⑶ 3 + ⑶ 8高细胞的增殖。虽然CD 3 + CD 8high细胞通常被认为是CTL，但直到49 dpi才在3头猪的PRRSV再刺激的PBMC中检测到CTL活性。仅在56 dpi时在一只猪的PRRSV再刺激的PBMC中观察到弱的PRRSV特异性CTL活性。相反，从21 dpi开始，在来自PRV海棠感染的猪的PRV海棠再刺激的PBMC中观察到明显的CTL活性。本研究表明，在体外再刺激下，PRRSV感染猪的PBMC含有增殖的CD 3 + CD 8high细胞，但这些PBMC不能对PRRSV感染的肺泡巨噬细胞发挥CTL活性。
The replication of porcine reproductive and respiratory syndrome virus (PRRSV) in lungs and lymphoid tissues of PRRSV-infected pigs is already strongly reduced before the appearance of neutralizing antibodies, indicating that other immune mechanisms are involved in eliminating PRRSV at those sites. This study aimed to determine whether PRRSV Lelystad virus (LV)-specific cytotoxic T-lymphocytes (CTL) can efficiently eliminate PRRSV-infected alveolar macrophages. Therefore, CTL assays were performed with PRRSV-infected alveolar macrophages as target cells and autologous peripheral blood mononuclear cells (PBMC) from PRRSV-infected pigs as a source of PRRSV-specific CTL. PBMC of 3 PRRSV-infected pigs were used either directly in CTL assays, or following restimulation in vitro. CTL assays with pseudorabies virus (PRV) Begonia-infected alveolar macrophages and autologous PBMC, from 2 PRV Begonia-inoculated pigs, were performed for validation of the assays. In freshly isolated PBMC, derived from PRRSV-infected pigs, CTL activity towards PRRSV-infected macrophages was not detected until the end of the experiment (56 days post infection – dpi). Restimulating the PBMC with PRRSV in vitro resulted in proliferation of CD3+CD8high cells starting from 14 dpi. Although CD3+CD8high cells are generally considered to be CTL, CTL activity was not detected in PRRSV-restimulated PBMC of the 3 pigs until 49 dpi. A weak PRRSV-specific CTL activity was observed only at 56 dpi in PRRSV-restimulated PBMC of one pig. In contrast, a clear CTL activity was observed in PRV Begonia-restimulated PBMC, derived from PRV Begonia-infected pigs, starting from 21 dpi. This study indicates that PBMC of PRRSV-infected pigs contain proliferating CD3+CD8high cells upon restimulation in vitro, but these PBMC fail to exert CTL activity towards PRRSV-infected alveolar macrophages.
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