beta-1 Integrins mediate tumour cell adhesion to quiescent endothelial cells in vitro.
beta-1 Integrins mediate tumour cell adhesion to quiescent endothelial cells in vitro.
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DOI：
10.1038/bjc.1996.627
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发表时间：
1996-12
影响因子：
8.8
通讯作者：
Murray, JC
中科院分区：
文献类型：
--
作者：
Price, EA;Coombe, DR;Murray, JC
一些实体瘤的转移扩散被认为是依赖于肿瘤细胞粘附到血管内皮，然后外渗到周围组织。我们研究了β 1整合素在乳腺腺癌细胞系MDA-MB-231和黑色素瘤细胞系rmi -7951对静止人脐静脉内皮细胞（HUVEC）的体外粘附中的作用。在粘附实验过程中，观察到肿瘤细胞粘附在静止的HUVEC单层上，特别是在内皮细胞-细胞连接处。免疫组织化学显示这些部位的β 1整合素表达浓度。用抗- 1整合素抗体预处理肿瘤细胞或HUVEC可减少粘附。用这些抗体同时治疗HUVECs和肿瘤细胞产生叠加性阻断效应，与异型粘附机制一致。我们的数据表明，在没有内皮“激活”的情况下，肿瘤细胞和内皮β 1整合素可能在肿瘤细胞通过血管内皮的阻滞和迁移中发挥关键作用。
Metastatic spread of some solid tumours is thought to depend upon the adhesion of tumour cells to the vascular endothelium followed by extravasation into surrounding tissues. We investigated the role of beta 1 integrins in the adhesion of the breast adenocarcinoma cell line MDA-MB-231 and the melanoma cell line RPMI-7951 to quiescent human umbilical vein endothelial cells (HUVEC) in vitro. In the course of adhesion assays, tumour cells were observed to adhere to quiescent HUVEC monolayers, particularly at endothelial cell-cell junctions. Immunohistochemistry revealed concentration of beta 1 integrin expression at these sites. Adhesion was reduced by pretreatment of either tumour cells or HUVEC with antibodies against beta 1 integrins. Simultaneous treatment of HUVECs and tumour cells with these antibodies produced an additive blocking effect, consistent with a heterotypic adhesion mechanism. Our data suggest that tumour cell and endothelial beta 1 integrins may play a crucial role in the arrest and migration of tumour cells through the vascular endothelium in the absence of endothelial 'activation'.
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影响因子：
15.9
作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
通讯作者：
MINICK, CR
影响因子：
3
作者：
DEJANA, E
通讯作者：
DEJANA, E
DOI：
10.1007/bf01621803
发表时间：
1993-03-01
期刊：
VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY
影响因子：
--
作者：
MECHTERSHEIMER, G;MUNK, M;MOLLER, P
通讯作者：
MOLLER, P
影响因子：
7.8
作者：
BORNSTEIN, P
通讯作者：
BORNSTEIN, P
影响因子：
4
作者：
MAEMURA, M;AKIYAMA, SK;DICKSON, RB
通讯作者：
DICKSON, RB