Agrobacterium-mediated transformation systems of Primula vulgaris.
Agrobacterium-mediated transformation systems of Primula vulgaris.
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DOI：
10.1186/s13007-018-0360-1
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发表时间：
2018
期刊：
影响因子：
5.1
通讯作者：
Gilmartin PM
中科院分区：
文献类型：
--
作者：
Hayta S;Smedley MA;Li J;Harwood WA;Gilmartin PM
遗传转化是植物功能基因组学研究中的一个重要步骤和重要工具，有助于发现基因，使人们能够深入了解基因功能和遗传控制的特性。报春花科物种提供了异形花发育的最著名的例子之一，这一育种系统引起了相当大的关注，包括查尔斯达尔文的研究。分子方法，包括植物转化提供了最好的机会，以确定和了解基因的作用，在共同的报春花，普通报春花，沿着与其他报春花属物种花异形。目前已建立了两种转化系统。第一个系统，农杆菌介导的真空渗透的幼苗，能够快速测试转基因，瞬时在植物。GUS基因在报春花幼苗的子叶、真叶和根中均有表达。第二个系统是基于根癌农杆菌（Agrobacteriumtumefaciens）感染的花梗外植体，平均转化效率为4.6%。该转化系统基于体外培养中转化体的再生和选择，证明了稳定的转基因整合和传递到下一代。这两个转化系统将有助于报春花重要性状的基础研究。虽然转基因的稳定整合是此类分析的最终目标，但是通过农杆菌介导的DNA转移的瞬时基因表达提供了分析转基因功能的简单且快速的方法。第二个系统描述了，第一次，稳定的农杆菌介导的转化报春花，这将是关键的特征基因负责控制花异形。
Genetic transformation is a valuable tool and an important procedure in plant functional genomics contributing to gene discovery, allowing powerful insights into gene function and genetically controlled characteristics. Primulaceae species provide one of the best-known examples of heteromorphic flower development, a breeding system which has attracted considerable attention, including that of Charles Darwin. Molecular approaches, including plant transformation give the best opportunity to define and understand the role of genes involved in floral heteromorphy in the common primrose, Primula vulgaris, along with other Primula species. Two transformation systems have been developed in P. vulgaris. The first system, Agrobacterium-mediated vacuum infiltration of seedlings, enables the rapid testing of transgenes, transiently in planta. GUS expression was observed in the cotyledons, true leaves, and roots of Primula seedlings. The second system is based on Agrobacterium tumefaciens infection of pedicel explants with an average transformation efficiency of 4.6%. This transformation system, based on regeneration and selection of transformants within in vitro culture, demonstrates stable transgene integration and transmission to the next generation. The two transformation systems reported here will aid fundamental research into important traits in Primula. Although, stable integration of transgenes is the ultimate goal for such analyses, transient gene expression via Agrobacterium-mediated DNA transfer, offers a simple and fast method to analyse transgene functions. The second system describes, for the first time, stable Agrobacterium-mediated transformation of Primula vulgaris, which will be key to characterising the genes responsible for the control of floral heteromorphy.
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影响因子：
5.2
作者：
Bandyopadhyay, S;Cane, K;Hamill, JD
通讯作者：
Hamill, JD
影响因子：
5.1
作者：
Li JF;Park E;von Arnim AG;Nebenführ A
通讯作者：
Nebenführ A
影响因子：
1.9
作者：
Hayta, Sadiye;Smedley, Mark A.;Gilmartin, Philip M.
通讯作者：
Gilmartin, Philip M.
影响因子：
3.7
作者：
GAMBORG, OL;MILLER, RA;OJIMA, K
通讯作者：
OJIMA, K
影响因子：
3
作者：
Fei, SZ;Read, PE;Riordan, TP
通讯作者：
Riordan, TP