Effect of cocaine on intracellular calcium regulation in myometrium from pregnant women.
Effect of cocaine on intracellular calcium regulation in myometrium from pregnant women.
复制标题
可卡因对孕妇子宫肌层细胞内钙调节的影响。
DOI：
10.1016/s1071-5576(99)00008-8
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Hurd,WW
中科院分区：
文献类型：
--
作者：
Fomin,VP;Singh,DM;Brown,HL;Natarajan,V;Hurd,WW
目的：通过确定可卡因可能调动的Ca2+的来源，评估可卡因对人子宫内膜细胞内游离钙（[Ca2+]i）调节的影响，并评估可卡因可能在儿茶酚胺对细胞[Ca2+]i的影响中所起的作用。方法：以孕妇子宫肌瘤细胞原代培养为实验模型。[Ca2+]对可卡因和去甲肾上腺素反应的相对变化用fura-2荧光法测定，并通过单向方差分析进行分析。结果：单独使用可卡因（10-8至10-3mol/L）可使[Ca2+]比基础水平增加43±18%，呈剂量依赖性。去甲肾上腺素也以浓度依赖的方式升高[Ca2+] i10 -4mol/L时（比基础水平高202±24%）。去甲肾上腺素引起的增加在无Ca2+介质中被抑制了48%，而可卡因的反应不受影响。Ca2+通道拮抗剂硝苯地平使[Ca2+]对10-5mol/L去甲肾上腺素的反应降低48%，而[Ca2+]对10-5mol/L可卡因的反应没有显著改变。肌浆网Ca2+泵抑制剂thapsigargin完全阻断了可卡因引起的[Ca2+]i的增加，而去甲肾上腺素反应则大大降低。同时，可卡因（10-8 ~ 10-3mol/L）不增强细胞中去甲肾上腺素引起的Ca2+]i的增加。结论：这些结果表明，可卡因增加[Ca2+]在怀孕的人子宫肌瘤细胞，主要是通过刺激Ca2+释放从囊内储存，而不是通过直接刺激Ca2+内流。
Objective:To evaluate the effect of cocaine on intracellular free calcium ([Ca2+]i) regulation in human myometrial cells by determining the sources of Ca2+it might mobilize, as well as assess the role cocaine might play in the catecholamine's effect on the cell's [Ca2+]i.Methods:Primary culture of myometrial cells from pregnant women was used as an experimental model. [Ca2+]irelative changes in response to cocaine and norepinephrine were measured with fura-2 fluorometry and analyzed bymeans of one-way analysisof variance.Results:Cocaine alone (10-8to 10-3mol/L) increased [Ca2+]iby up to 43 ± 18% over basal level in a dose-dependent manner. Norepinephrine also elevated [Ca2+]iin a concentration-dependent manner (202 ± 24% over basal level at 10-4mol/L). The norepinephrine-evoked increase was inhibited in Ca2+-free media by 48% whereas the cocaine response was not affected. The Ca2+-channel antagonist nifedipine caused decrease in the [Ca2+]iresponse to 10-5mol/L of norepinephrine by 48%, whereas the [Ca2+]irise to 10-5mol/L cocaine was not significantly chaned. Inhibitor of the sarcoplasmic reticulum Ca2+pump, thapsigargin, completely blocked cocaine-evoked increases in [Ca2+]i, whereas norepinephrine response were greatly reduced. At the same time, cocaine (10-8to 10-3mol/L) did not potentiate norepinephrine-evoked Ca2+]i, increases in the cells.Conclusion:These results indicate that cocaine increases [Ca2+]iin pregnant human myometrial cells, primarily by stimulating release of Ca2+from intracefullar stores rather than by direct stimulation of Ca2+influx.
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影响因子：
9.8
作者：
M. Monga;N. Weisbrodt;Robert L. Andres;Barbara M. Sanborn
通讯作者：
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影响因子：
9.8
作者：
Phillippe,M
通讯作者：
Phillippe,M
DOI：
--
发表时间：
1985-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Grynkiewicz;M. Poenie;Roger Y. TsienB
通讯作者：
G. Grynkiewicz;M. Poenie;Roger Y. TsienB
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影响因子：
2.1
作者：
E. Daniel;M. Wolowyk
通讯作者：
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DOI：
10.1016/0006-291x(85)91718-8
发表时间：
1985
影响因子：
3.1
作者：
M. E. Carsten;J. Miller
通讯作者：
J. Miller