BDNF is required for taste axon regeneration following unilateral chorda tympani nerve section.
BDNF is required for taste axon regeneration following unilateral chorda tympani nerve section.
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DOI：
10.1016/j.expneurol.2017.03.016
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发表时间：
2017-07
影响因子：
5.3
通讯作者：
Krimm R
中科院分区：
文献类型：
--
作者：
Meng L;Huang T;Sun C;Hill DL;Krimm R
味觉神经很容易再生去神经支配的味蕾，然而，再生所需的因素尚未确定。当鼓索神经被切断，表达脑源性神经营养因子（BDNF）仍然高膝状神经节和舌上皮，尽管味蕾的损失。这些观察结果表明，BDNF存在于神经切断后的味觉系统中，并可能支持味觉神经再生。为了验证这一假设，我们在成年小鼠中诱导性地删除了BDNF。BDNF基因重组后不久，鼓索神经被单侧切断，导致味蕾和神经元的损失，与BDNF水平无关。然而，神经切片后8周，BDNF缺失对再生的影响不同。在对照组小鼠中，鼓索神经再生，味蕾重新出现神经支配。相反，在缺乏正常BDNF表达的小鼠中，很少有味蕾被重新神经支配，使得味蕾数量保持较低。在所有的基因型中，重新神经支配的味蕾是正常大小的，但非神经支配的味蕾仍然很小，萎缩。在神经部分对侧的舌头一侧，一些基因型的味蕾变得更大，所有的味蕾仍然受神经支配。我们的研究结果表明，脑源性神经营养因子是所需的神经再生后的味觉神经部分。
Taste nerves readily regenerate to reinnervate denervated taste buds; however, factors required for regeneration have not yet been identified. When the chorda tympani nerve is sectioned, expression of brain-derived neurotrophic factor (BDNF) remains high in the geniculate ganglion and lingual epithelium, despite the loss of taste buds. These observations suggest that BDNF is present in the taste system after nerve section and may support taste nerve regeneration. To test this hypothesis, we inducibly deleted Bdnf during adulthood in mice. Shortly after Bdnf gene recombination, the chorda tympani nerve was unilaterally sectioned causing a loss of both taste buds and neurons, irrespective of BDNF levels. Eight weeks after nerve section, however, regeneration was differentially affected by Bdnf deletion. In control mice, there was regeneration of the chorda tympani nerve and taste buds reappeared with innervation. In contrast, few taste buds were reinnervated in mice lacking normal Bdnf expression such that taste bud number remained low. In all genotypes, taste buds that were reinnervated were normal-sized, but non-innervated taste buds remained small and atrophic. On the side of the tongue contralateral to the nerve section, taste buds for some genotypes became larger and all taste buds remained innervated. Our findings suggest that BDNF is required for nerve regeneration following gustatory nerve section.
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影响因子：
3.3
作者：
He, L.;Yadgarov, A.;Sharif, S.;McCluskey, L. P.
通讯作者：
McCluskey, L. P.
影响因子：
2.5
作者：
Ganchrow, D;Ganchrow, JR;Whitehead, MC
通讯作者：
Whitehead, MC
DOI：
10.1152/ajpregu.00219.2009
发表时间：
2009-10-01
影响因子：
2.8
作者：
Guagliardo, Nick A.;West, Katie Nicole;Hill, David L.
通讯作者：
Hill, David L.
影响因子：
4.1
作者：
Gordon, Tessa
通讯作者：
Gordon, Tessa
影响因子：
2.5
作者：
Guagliardo, Nick A.;Hill, David L.
通讯作者：
Hill, David L.