Saliva as a medium to detect and measure biomarkers related to pain.
Saliva as a medium to detect and measure biomarkers related to pain.
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DOI：
10.1038/s41598-018-21131-4
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发表时间：
2018-02-19
影响因子：
4.6
通讯作者：
Ernberg M
中科院分区：
文献类型：
--
作者：
Jasim H;Carlsson A;Hedenberg-Magnusson B;Ghafouri B;Ernberg M
唾液作为一种具有诊断或预后价值的体液，尽管被患者普遍接受，但往往被忽视。这是由于缺乏标准化的收集程序。本研究的目的是确定理想的唾液收集技术，并开发新的敏感方法来检测和分析健康无痛受试者的疼痛相关标志物。在严格控制的条件下评价血浆和五种不同的唾液收集方法。采用新的基于蛋白质印迹的技术测定神经生长因子（NGF）、降钙素基因相关肽（CGRP）和脑源性神经营养因子（BDNF）的水平。使用市售方法测定谷氨酸盐和P物质（SP）。在唾液中发现了几种新的NGF、CGRP和BDNF亚型。同种型模式显示了不同收集方法之间的表达和化学发光水平的显著变化。本研究开发了一种新的敏感方法来研究唾液中与疼痛相关的标志物。此外，我们首次证明了刺激的全唾液和血液中谷氨酸浓度之间的相关性。然而，得出的基本结论是收集方法一致性的重要性。
Saliva is often neglected as a body fluid of diagnostic or prognostic value, even though generally well accepted by the patients. This is due to lack of a standardized collection procedure. The aim of this study was to identify the ideal saliva collection technique and develop new sensitive methods to detect and analyse markers related to pain in healthy pain-free subjects. Plasma and five different saliva collection approached was evaluated during strictly controlled conditions. Levels of nerve growth factor (NGF), calcitonin gene-related peptide (CGRP) and brain derived neurotropic factor (BDNF) were determined using novel western blotting based technology. Glutamate and substance P (SP) was determined using commercial available methods. Several new isoforms were found for NGF, CGRP and BDNF in saliva. The isoform pattern showed significant variation in both expression and chemiluminescence levels between different collection methods. New sensitive methods to study pain related markers in saliva were developed in this study. Furthermore, we are first to demonstrate a correlation between the Glutamate concentration in stimulated whole saliva and blood. However, the fundamental conclusion drawn is the importance of consistency in the collection method.
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影响因子：
2.9
作者：
GOEDERT, M;NAGY, JI;EMSON, PC
通讯作者：
EMSON, PC
影响因子：
7.4
作者：
Fischer, Marlene;Wille, Georg;Klien, Stephanie;Shanib, Hind;Holle, Dagny;Gaul, Charly;Broessner, Gregor
通讯作者：
Broessner, Gregor
影响因子：
2.5
作者：
Schiffman E;Ohrbach R;Truelove E;Look J;Anderson G;Goulet JP;List T;Svensson P;Gonzalez Y;Lobbezoo F;Michelotti A;Brooks SL;Ceusters W;Drangsholt M;Ettlin D;Gaul C;Goldberg LJ;Haythornthwaite JA;Hollender L;Jensen R;John MT;De Laat A;de Leeuw R;Maixner W;van der Meulen M;Murray GM;Nixdorf DR;Palla S;Petersson A;Pionchon P;Smith B;Visscher CM;Zakrzewska J;Dworkin SF;International RDC/TMD Consortium Network, International association for Dental Research;Orofacial Pain Special Interest Group, International Association for the Study of Pain
通讯作者：
Orofacial Pain Special Interest Group, International Association for the Study of Pain
影响因子：
3.8
作者：
Nam, J-W;Chung, J-W;Kim, Y-K
通讯作者：
Kim, Y-K
DOI：
10.1196/annals.1384.033
发表时间：
2007-01-01
期刊：
ORAL-BASED DIAGNOSTICS
影响因子：
--
作者：
Amerongen, Arie v. Nieuw;Ligtenberg, Antoon J. M.;Veerman, Enno C. I.
通讯作者：
Veerman, Enno C. I.