Global analysis of phase variation in Myxococcus xanthus.
Global analysis of phase variation in Myxococcus xanthus.
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DOI：
10.1111/j.1365-2958.2011.07732.x
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发表时间：
2011-08
影响因子：
3.6
通讯作者：
Hartzell P
中科院分区：
文献类型：
--
作者：
Furusawa G;Dziewanowska K;Stone H;Settles M;Hartzell P
Myxococcus xanthus can vary its phenotype or “phase” to produce colonies that contain predominantly yellow or tan cells that differ greatly in their abilities to swarm, survive, and develop. Yellow variants are proficient at swarming (++) and tend to lyse in liquid during stationary phase. In contrast, tan variants are deficient in swarming (+) and persist beyond stationary phase. The phenotypes and transcriptomes of yellow and tan variants were compared with mutants affected in phase variation. Thirty-seven genes were upregulated specifically in yellow variants including those for production of the yellow pigment, DKxanthene. A mutant in DKxanthene synthesis produced nonpigmented (tan) colonies but still phase varied for swarming suggesting that pigmentation is not the cause of phase variation. Disruption of a gene encoding a HTH-Xre-like regulator, highly expressed in yellow variants, abolished pigment production and blocked the ability of cells to switch from a swarm ++ to a swarm (+) phenotype, showing that HTH-Xre regulates phase variation. Among the four genes whose expression was increased in tan variants was pkn14, which encodes a serine-threonine kinase that regulates programmed cell death in Myxococcus via the MrpC-MazF toxin-antitoxin complex. High levels of phosphorylated Pkn14 may explain why tan cells enjoy enhanced survival.
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影响因子：
10.7
作者：
Kadam, Supriya V.;Wegener-Feldbruegge, Sigrun;Velicer, Gregory J.
通讯作者：
Velicer, Gregory J.
影响因子：
3.2
作者：
Calvio, Cinzia;Osera, Cecilia;Galizzi, Alessandro
通讯作者：
Galizzi, Alessandro
影响因子：
11.4
作者：
Lampe, DJ;Churchill, MEA;Robertson, HM
通讯作者：
Robertson, HM
影响因子：
3.2
作者：
Britton, RA;Eichenberger, P;Grossman, AD
通讯作者：
Grossman, AD
影响因子：
64.5
作者：
KIM, SK;KAISER, D
通讯作者：
KAISER, D