Genome-wide transcription analysis of clinal genetic variation in Drosophila.
Genome-wide transcription analysis of clinal genetic variation in Drosophila.
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DOI：
10.1371/journal.pone.0034620
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Partridge L
中科院分区：
文献类型：
--
作者：
Chen Y;Lee SF;Blanc E;Reuter C;Wertheim B;Martinez-Diaz P;Hoffmann AA;Partridge L
数量性状的临床变异很普遍，但其遗传基础有待鉴定。果蝇在多个大陆上沿纬度梯度的体型等性状表现出适应性的临床变异。为了研究可能导致临床表型变异的南北基因组范围内的转录差异，我们使用全基因组平铺阵列比较了来自热带北部和温带南部种群的黑腹果蝇发育过程中的RNA表达模式。我们发现，在细胞末端之间差异表达的基因通常与新陈代谢和生长有关，并且对它们样本的表达进行实验性改变通常会导致身体尺寸在预测方向上发生改变，有时甚至是显着改变。我们进一步确定了蛇（srp）转录因子结合位点在南部表达上调的基因附近富集。临床群体分析揭示了 srp 表达水平的显着变化。 srp 的实验过度表达增加了体型，正如从其临床表达模式预测的那样，表明它可能参与调节果蝇的适应性临床变异。这项研究确定了一些通过改变基因表达水平导致临床表型变异的基因，但个别基因的错误表达导致了适度的体型变化。
Clinal variation in quantitative traits is widespread, but its genetic basis awaits identification. Drosophila melanogaster shows adaptive, clinal variation in traits such as body size along latitudinal gradients on multiple continents. To investigate genome wide transcription differentiation between North and South that might contribute to the clinal phenotypic variation, we compared RNA expression patterns during development of D. melanogaster from tropical northern and temperate southern populations using whole genome tiling arrays. We found that genes that were differentially expressed between the cline ends were generally associated with metabolism and growth, and experimental alteration of expression of a sample of them generally resulted in altered body size in the predicted direction, sometimes significantly so. We further identified the serpent (srp) transcription factor binding sites to be enriched near genes up-regulated in expression in the south. Analysis of clinal populations revealed a significant cline in the expression level of srp. Experimental over-expression of srp increased body size, as predicted from its clinal expression pattern, suggesting that it may be involved in regulating adaptive clinal variation in Drosophila. This study identified a handful of genes that contributed to clinal phenotypic variation through altered gene expression level, yet misexpression of individual gene led to modest body size change.
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DOI：
10.1073/pnas.85.14.5161
发表时间：
1988-07-01
影响因子：
11.1
作者：
LAURIE, CC;STAM, LF
通讯作者：
STAM, LF
影响因子：
4.8
作者：
LIN, WH;HUANG, LH;TSAI, SF
通讯作者：
TSAI, SF
影响因子：
64.8
作者：
Gompel, N;Prud'homme, B;Carroll, SB
通讯作者：
Carroll, SB
影响因子：
11.4
作者：
Petersen, UM;Kadalayil, L;Engström, Y
通讯作者：
Engström, Y
影响因子：
3.3
作者：
Kennington, W. Jason;Hoffmann, Ary A.;Partridge, Linda
通讯作者：
Partridge, Linda