Development and migration of plasma cells in the mouse lymph node.
Development and migration of plasma cells in the mouse lymph node.
复制标题
DOI：
10.1016/j.immuni.2010.06.015
复制
发表时间：
2010-07-23
期刊：
影响因子：
32.4
通讯作者：
Skokos D
中科院分区：
文献类型：
--
作者：
Fooksman DR;Schwickert TA;Victora GD;Dustin ML;Nussenzweig MC;Skokos D
在这项研究中，我们用活体显微镜观察了小鼠淋巴结新生浆细胞（PCs）的分化和迁移行为。前pc表现出一种独特的迁移模式，其特征是随机定向的长线性路径。虽然通过Gαi偶联受体的趋化性与PC迁移有关，但百日咳毒素（Ptx）治疗可以消除这些信号，并不能阻止前PC的移动，但它可以阻止其他淋巴细胞。在体外，预分化的PC在涂有整合素配体的表面上表现出进步性变形虫运动，而完全分化的PC则移动缓慢或停滞。髓索内均发生PC停搏和分化。PC分化前的Ptx治疗阻断了其在髓索的积累，但PC分化前仍在其他淋巴结区分化。综上所述，我们建议pre-PCs通过持续的随机游走来找到髓索，在髓索中，局部趋化因子帮助保留这些细胞，直到它们分化并原地停止。
In this study, we imaged the differentiation and migratory behavior of nascent plasma cells (PCs) in mouse lymph nodes by intravital microscopy.. Pre-PCs exhibited a unique migration pattern characterized by long, linear paths that were randomly oriented. Although chemotaxis via Gαi coupled-receptors has been implicated in PC migration, treatment with Pertussis toxin (Ptx), which ablates these signals, did not prevent movement of pre-PCs while it arrested other lymphocytes. In vitro, pre-PCs displayed processive amoeboid locomotion on surfaces coated with integrin ligand, while fully-differentiated PC moved slowly or were arrested. Both PC arrest and differentiation occurred in the medullary cords. Ptx treatment before PC differentiation blocked their accumulation in the medullary cords but pre-PCs still differentiated in other lymph node regions. Taken together, we suggest pre-PCs undergo a persistent random walk to find the medullary cords, where localized chemokines help retain these cells until they undergo differentiation and arrest in situ.
登录
查看更多内容
影响因子：
32.4
作者：
Rutishauser, Rachel L.;Martins, Gislaine A.;Kalachikov, Sergey;Chandele, Anmol;Parish, Ian A.;Meffre, Eric;Jacob, Joshy;Calame, Kathryn;Kaech, Susan M.
通讯作者：
Kaech, Susan M.
影响因子：
56.9
作者：
Allen, Christopher D. C.;Okada, Takaharu;Cyster, Jason G.
通讯作者：
Cyster, Jason G.
影响因子：
4.4
作者：
Hauser, AE;Debes, GF;Manz, RA
通讯作者：
Manz, RA
DOI：
10.1084/jem.194.1.45
发表时间：
2001-07-02
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hargreaves DC;Hyman PL;Lu TT;Ngo VN;Bidgol A;Suzuki G;Zou YR;Littman DR;Cyster JG
通讯作者：
Cyster JG
影响因子：
4.4
作者：
Huang, Julie H.;Cardenas-Navia, L. Isabel;Dustin, Michael L.
通讯作者：
Dustin, Michael L.