A hyaluronic acid granular hydrogel nerve guidance conduit promotes regeneration and functional recovery of injured sciatic nerves in rats.
A hyaluronic acid granular hydrogel nerve guidance conduit promotes regeneration and functional recovery of injured sciatic nerves in rats.
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DOI：
10.4103/1673-5374.350212
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发表时间：
2023-03
影响因子：
6.1
通讯作者：
Li YQ
中科院分区：
文献类型：
--
作者：
Yang J;Hsu CC;Cao TT;Ye H;Chen J;Li YQ
透明质酸颗粒水凝胶可以促进体外神经元和星形胶质细胞集落形成和轴突延伸，表明水凝胶可以模拟细胞外基质结构以促进神经再生。然而，尚未进行体内实验。在这项研究中，我们移植了透明质酸颗粒水凝胶神经引导导管，以修复10毫米长的坐骨神经缺损。Basso、Beattie和Bresnahan运动评定量表、坐骨神经复合肌肉动作电位记录、Fluoro-Gold逆行追踪、生长相关蛋白43/S100免疫荧光染色、透射电镜、腓肠肌干/湿重比和Masson三色染色结果表明，神经引导导管表现出相似的坐骨神经轴突和髓鞘再生，以及与自体神经移植一样恢复电生理功能和运动功能。导管结果上级优于散装水凝胶或硅胶管移植。这些结果表明，含有透明质酸颗粒水凝胶的组织工程神经导管有效地促进了损伤坐骨神经的形态和功能恢复。神经导管具有作为周围神经缺损修复材料的潜力。
A hyaluronic acid granular hydrogel can promote neuronal and astrocyte colony formation and axonal extension in vitro, suggesting that the hydrogel can simulate an extracellular matrix structure to promote neural regeneration. However, in vivo experiments have not been conducted. In this study, we transplanted a hyaluronic acid granular hydrogel nerve guidance conduit to repair a 10-mm long sciatic nerve gap. The Basso, Beattie, and Bresnahan locomotor rating scale, sciatic nerve compound muscle action potential recording, Fluoro-Gold retrograde tracing, growth related protein 43/S100 immunofluorescence staining, transmission electron microscopy, gastrocnemius muscle dry/wet weight ratio, and Masson’s trichrome staining results showed that the nerve guidance conduit exhibited similar regeneration of sciatic nerve axons and myelin sheath, and recovery of the electrophysiological function and motor function as autologous nerve transplantation. The conduit results were superior to those of a bulk hydrogel or silicone tube transplant. These findings suggest that tissue-engineered nerve conduits containing hyaluronic acid granular hydrogels effectively promote the morphological and functional recovery of the injured sciatic nerve. The nerve conduits have the potential as a material for repairing peripheral nerve defects.
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一种用于修复周围神经损伤的新型生物活性神经导管
影响因子：
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作者：
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