Quick-Freeze, Deep-Etch Electron Microscopy Reveals the Characteristic Architecture of the Fission Yeast Spore.
Quick-Freeze, Deep-Etch Electron Microscopy Reveals the Characteristic Architecture of the Fission Yeast Spore.
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DOI：
10.3390/jof7010007
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发表时间：
2020-12-26
期刊：
影响因子：
--
通讯作者：
Nakamura T
中科院分区：
文献类型：
--
作者：
Tahara YO;Miyata M;Nakamura T
裂殖酵母的孢子是一种休眠细胞，能抵抗各种环境压力。色葡萄粟酒裂殖酵母孢子被蛋白质表面层包被，由于其主要包含Isp 3蛋白，因此被称为Isp 3层。虽然薄切片电子显微镜和扫描电子显微镜已经揭示了孢子的基本结构，但其结构仍不清楚。在这里，我们可视化S。粟酒裂殖酵母孢子通过使用快速冷冻复制电子显微镜（QFDE-EM）在纳米分辨率，揭示了新的特征结构。QFDE-EM揭示了Isp 3层作为交织的纤维层存在。在孢子细胞膜上，许多比营养细胞膜上的内陷更长的深内陷平行排列。我们还观察到，在孢子萌发过程中，细胞表面的变化从光滑的树枝状丝状结构，后者是营养细胞的特征。这些发现不仅为孢子的结构组成，而且还为细胞的应激反应机制提供了重要的见解。
The spore of the fission yeast Schizosaccharomyces pombe is a dormant cell that is resistant to a variety of environmental stresses. The S. pombe spore is coated by a proteinaceous surface layer, termed the Isp3 layer because it comprises mainly Isp3 protein. Although thin-section electron microscopy and scanning electron microscopy have revealed the fundamental structure of the spore, its architecture remains unclear. Here we visualized S. pombe spores by using a quick-freeze replica electron microscopy (QFDE-EM) at nanometer resolution, which revealed novel characteristic structures. QFDE-EM revealed that the Isp3 layer exists as an interwoven fibrillar layer. On the spore cell membrane, many deep invaginations, which are longer than those on the vegetative cell membrane, are aligned in parallel. We also observed that during spore germination, the cell surface changes from a smooth to a dendritic filamentous structure, the latter being characteristic of vegetative cells. These findings provide significant insight into not only the structural composition of the spore, but also the mechanism underlying the stress response of the cell.
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DOI：
10.1083/jcb.81.2.275
发表时间：
1979-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heuser JE;Reese TS;Dennis MJ;Jan Y;Jan L;Evans L
通讯作者：
Evans L
影响因子：
3.6
作者：
Arellano, M;Cartagena-Lirola, H;Valdivieso, MH
通讯作者：
Valdivieso, MH
影响因子：
3.3
作者：
Neiman, Aaron M.
通讯作者：
Neiman, Aaron M.
裂殖酵母孢子被主要包含Isp3的蛋白质表面层覆盖。
影响因子：
3.3
作者：
Fukunishi K;Miyakubi K;Hatanaka M;Otsuru N;Hirata A;Shimoda C;Nakamura T
通讯作者：
Nakamura T
影响因子：
3.7
作者：
EGEL, R;EGELMITA.M
通讯作者：
EGELMITA.M