Misdirection of dosage compensation underlies bidirectional sex-specific death in Wolbachia-infected Ostrinia scapulalis.
Misdirection of dosage compensation underlies bidirectional sex-specific death in Wolbachia-infected Ostrinia scapulalis.
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剂量补偿的误导是沃尔巴克氏体感染的肩胛玉米螟双向性别特异性死亡的基础。
DOI：
10.1016/j.ibmb.2015.10.001
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发表时间：
2015
影响因子：
3.8
通讯作者：
T.Tsuchida
中科院分区：
文献类型：
--
作者：
T.N.Sugimoto;T.Kayukawa;T.Shinoda;Y.Ishikawa;T.Tsuchida
沃尔巴克氏菌属的内共生细菌经常操纵宿主的生殖系统，在宿主种群中繁殖自己。感染沃尔巴克氏体（wolbachia, wSca）的肩胛飞蛾只产生雌性后代（性染色体：ZW），而经抗生素治疗治愈的雌性只产生雄性后代（ZZ）。只有雌性和雄性后代的发生归因于异性在胚胎和幼虫发育过程中的特定死亡。在这种双向性别特异性致死性中，注定死亡的胚胎表达与其基因型性别相反的表型性别。基于这些发现，我们认为，由于携带了一种使雄性宿主雌性化的遗传因素，宿主的W染色体失去了雌性化功能，基因型和表型性别之间的不一致是这种性别特异性死亡的基础。在目前的研究中，我们研究了剂量补偿的失败是否是造成这种性别特异性死亡率的原因。定量pcr结果显示，死亡胚胎中z连锁基因的表达水平没有得到适当的剂量补偿；感染了wsca的雌性的雄性后代大约高两倍，而感染和治愈的雌性的雌性后代大约低两倍。这些结果支持了我们的假设，即剂量补偿的误导是性别特异性死亡的基础。
Endosymbiotic bacteria of the genus Wolbachia often manipulate the reproductive system of their hosts to propagate themselves in host populations. Ostrinia scapulalis moths infected withWolbachia(wSca) produce female-only progeny (sex chromosomes: ZW), whereas females cured of the infection by antibiotic treatment produce male-only progeny (ZZ). The occurrence of female- and male-only progeny has been attributed to the specific death of the opposite sex during embryonic and larval development. In this bidirectional sex-specific lethality, embryos destined to die express a phenotypic sex opposite to their genotypic sex. On the basis of these findings, we suggested thatwScacarries a genetic factor that feminizes the male host, the W chromosome of the host has lost its feminizing function, and discordance between the genotypic and phenotypic sexes underlies this sex-specific death. In the present study, we examined whether the failure of dosage compensation was responsible for this sex-specific mortality. Quantitative PCRs showed that Z-linked gene expression levels in embryos destined to die were not properly dosage compensated; they were approximately two-fold higher in the male progeny ofwSca-infected females and approximately two-fold lower in the female progeny of infected-and-cured females. These results support our hypothesis that misdirection of dosage compensation underlies the sex-specific death.
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