Intranasal immunization of the combined lipooligosaccharide conjugates protects mice from the challenges with three serotypes of Moraxella catarrhalis.
Intranasal immunization of the combined lipooligosaccharide conjugates protects mice from the challenges with three serotypes of Moraxella catarrhalis.
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DOI：
10.1371/journal.pone.0029553
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Gu XX
中科院分区：
文献类型：
--
作者：
Ren D;Xie H;Zhang W;Hassan F;Petralia RS;Yu S;Lim DJ;Gu XX
目前还没有针对卡他莫拉菌（一种重要的人类呼吸道病原体）的许可疫苗。来自单个血清型M的基于脂寡糖（LOS）的结合疫苗。卡他炎仅显示出部分保护覆盖。结合两种或三种血清型的LOS结合物的疫苗可能提供更广泛的保护。用由来自血清型A和B或血清型A、B和C的LOS组成的组合缀合物鼻内免疫小鼠，然后用不同的M攻击。三种血清型的卡他菌株。每次激发后收集小鼠肺、鼻洗液和血清，用于细菌计数、组织学评价、细胞因子谱、抗体水平和结合活性测定。鼻内给予组合LOS缀合物不仅增强了所有三种血清型M.接种小鼠中的卡他菌株，而且分别在鼻洗液和血清中升高了型特异性抗LOS免疫球蛋白（IG）A和IgG滴度。用组合的LOS缀合物接种的小鼠在攻击后还显示肺中的干扰素（IFN）-γ、白细胞介素（IL）-12和IL-4增加。与对照组相比，用组合的LOS缀合物免疫的小鼠在M.粘膜炎感染。结合物诱导的超免疫血清对所有三种血清型的M都表现出广泛的交叉反应性。透射电子显微镜下的粘膜炎。血清型A和B LOS结合物的联合疫苗提供针对大多数M的保护。卡他菌菌株通过引发体液和细胞免疫应答。
There are no licensed vaccines available against Moraxella catarrhalis, a significant human respiratory pathogen. Lipooligosaccharide (LOS) based conjugate vaccines derived from individual serotype M. catarrhalis only showed partial protection coverage. A vaccine combining LOS conjugates of two or three serotypes might provide a broader protection. Mice were immunized intranasally with the combined conjugates consisting of LOS from serotype A and B or serotype A, B, and C followed by challenge with different M. catarrhalis strains of three serotypes. Mouse lungs, nasal washes, and sera were collected after each challenge for bacterial counts, histological evaluation, cytokine profiles, antibody level and binding activity determinations. Intranasal administration of the combined LOS conjugates not only enhanced pulmonary bacterial clearance of all three serotypes of M. catarrhalis strains in vaccinated mice, but also elevated serotype-specific anti-LOS immunoglobulin (Ig)A and IgG titers in nasal wash and serum respectively. Mice vaccinated with the combined LOS conjugates also showed increased interferon (IFN)-γ, interleukin (IL)-12, and IL-4 in the lungs after challenges. Compared to the control group, mice immunized with the combined LOS conjugates also showed reduced lung inflammation after M. catarrhalis infections. The hyperimmune sera induced by the combined conjugates exhibited a broad cross-reactivity toward all three serotypes of M. catarrhalis under transmission electron microscopy. The combined vaccine of serotype A and B LOS conjugates provides protection against most M. catarrhalis strains by eliciting humoral and cellular immune responses.
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影响因子：
11.8
作者：
Murphy, Timothy F.;Parameswaran, G. Iyer
通讯作者：
Parameswaran, G. Iyer
影响因子：
3.1
作者：
EDEBRINK, P;JANSSON, PE;WEINTRAUB, A
通讯作者：
WEINTRAUB, A
影响因子：
9.4
作者：
VANEECHOUTTE, M;VERSCHRAEGEN, G;VANDENABEELE, AM
通讯作者：
VANDENABEELE, AM
影响因子：
3.1
作者：
Aebi, C;Cope, LD;Hansen, EJ
通讯作者：
Hansen, EJ
影响因子：
3.1
作者：
Jiao, XN;Hirano, T;Gu, XX
通讯作者：
Gu, XX