Exploring the neonatal salivary transcriptome: technical optimization and clinical applications.
Exploring the neonatal salivary transcriptome: technical optimization and clinical applications.
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DOI：
10.1016/j.clinbiochem.2011.03.019
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发表时间：
2011-05-01
影响因子：
2.8
通讯作者：
Maron JL
中科院分区：
文献类型：
--
作者：
Maron JL
方法从新生儿唾液转录组、系列全唾液样本中检测生物学相关信息（50-200 μL）来自早产儿（n= 5;胎龄28 - 32周）在其住院期间的五个不同时间点收集：1）出生后不久肠内营养前; 2）肠内营养开始时; 3）在推进肠内营养期间; 4）在开始经口喂养时;以及5）在完全或接近完全经口喂养时。提取总RNA，扩增并在每个时间点的每个婴儿的Affyphase HG U133 2.0 Plus全基因组微阵列上杂交（n= 25）。将阵列归一化并进行生物信息学分析，以鉴定随时间推移在统计学上显著改变其表达的基因。鉴定的基因通过Incidity ® Pathway Analyses（IPA）进行进一步分析，以更好地了解基因-基因关系和与早产儿正常生理发育相关的重要生物学途径。
MethodsTo examine biologically relevant information derived from the neonatal salivary transcriptome, serial whole saliva samples (50–200 μL) from premature infants (n= 5; gestational age 28 to 32 weeks) were collected during five distinct time points during their hospitalization: 1) shortly after birth prior to enteral nutrition; 2) at the start of enteral nutrition; 3) during the advancement of enteral nutrition; 4) at the start of oral feeding; and 5) at full or near full oral feeds. Total RNA was extracted, amplified and hybridized onto Affymetrix HG U133 2.0 Plus whole genomic microarrays for each baby for each time point (n= 25). Arrays were normalized and underwent bioinformatic analyses to identify genes that were statistically significantly altering their expression over time. Genes identified underwent further analyses with the Ingenuity® Pathway Analyses (IPA) to better understand gene-gene relationships and important biological pathways associated with the normal physiological development of premature infants.
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影响因子：
11.5
作者：
Li, Y;St John, MAR;Wong, DT
通讯作者：
Wong, DT
影响因子：
2.4
作者：
Segal, Alik;Wong, David T.
通讯作者：
Wong, David T.
影响因子：
--
作者：
Wong, David T.
通讯作者：
Wong, David T.
影响因子：
9.3
作者：
Maron, Jill L.;Johnson, Kirby L.;Bianchi, Diana W.
通讯作者：
Bianchi, Diana W.