Expression and function of a new angiogenic factor AA98 target molecule at the maternal-embryonic boundary of rhesus monkey
Expression and function of a new angiogenic factor AA98 target molecule at the maternal-embryonic boundary of rhesus monkey
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新型血管生成因子AA98靶分子在恒河猴母体-胚胎边界的表达和功能
DOI：
10.1007/bf03325669
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发表时间：
2003-05
期刊：
影响因子：
--
通讯作者：
Yan, XY
中科院分区：
文献类型：
--
作者：
Zhang, ZH;Wei, P;Liu, YX;Chen, XL;Yan, XY
单克隆抗体AA 98（简称AA）的靶分子是一种新的血管内皮细胞相关因子，已有资料表明其在血管生成中发挥作用。为了探讨其在灵长类动物血管生成和胎盘形成中的作用，我们采用免疫组化和Western免疫印迹法检测了其在恒河猴妊娠D17、19、28和34着床部位的表达。Western blot分析表明，本研究中使用的一抗对其表位具有特异性。AA蛋白主要表达于小血管和部分细胞滋养层细胞。AA染色主要见于内皮细胞和血管小肌。这一观察结果支持AA在血管生成中的作用。AA在细胞滋养层中的时空表达：在细胞柱内的增殖滋养层和血管内滋养层中较弱，在基板内的滋养层亚群和血管滋养层中较强;在胎盘形成早期，基板内的AA染色下调。AA 98在绒毛外滋养层细胞表达的变化表明了这种新的因子在细胞滋养层转移和螺旋动脉重塑过程中的作用。AA 98的时空表达表明AA 98也可以作为滋养层细胞标志物来表征血管内皮和侵袭表型的获得。
The target molecule of monoclonal antibody AA98 (AA for short) is a new vascular endothelial cell related factor and plays a role in angiogenesis as indicated by the previous data. To investigate its role in angiogenesis and placentation in primate, we examined its expression in the implantation sites on D17, 19, 28 and 34 of gestation in rhesus monkey by immunohistochemistry and Western immunoblot. Western blot analysis showed that the primary antibody used in this study was specific for its epitope. AA protein was mainly expressed in small blood vessels and in some cytotrophoblast cells. The AA staining was found mainly in the endothelial cells and vascular small muscle. This observation supported the AA's role in angiogenesis. AA was spatio-temporarily expressed in cytotrophoblasts: weak in proliferating trophoblast within cell column and endovascular trophoblast, strong in trophoblastic subpopulation within the basal plate and vascular trophoblast; AA staining within the basal plate was down-regulated during early placentation. The shift of AA98 expression in extravillous trophoblasts suggestes a role of this new factor during the course of cytotrophoblast metastasis and spiral artery remodeling. The spatio-temporarily expression indicats that AA98 could be also used as a trophoblast cellular marker to characterize the acquisition of a vascular endothelial and invasive phenotype.
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DOI：
10.1177/107155769900600610
发表时间：
1999-11
期刊：
Journal of the Society for Gynecologic Investigation
影响因子：
--
作者：
Xiangyun Zhang;R. Pettengell;N. Nasiri;V. Kalia;A. Dalgleish;D. Barton
通讯作者：
Xiangyun Zhang;R. Pettengell;N. Nasiri;V. Kalia;A. Dalgleish;D. Barton
影响因子：
3.6
作者：
L. Reynolds;J. Kirsch;K. C. Kraft;D. Redmer
通讯作者：
L. Reynolds;J. Kirsch;K. C. Kraft;D. Redmer
DOI：
--
发表时间：
1997
期刊：
--
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.6
作者：
Samathanam, CA;Adesanya, OO;Bondy, CA
通讯作者：
Bondy, CA
影响因子：
--
作者：
Hongjuan Gao;Xinlei Chen;Lei Shi;Yi-Xun Liu
通讯作者：
Hongjuan Gao;Xinlei Chen;Lei Shi;Yi-Xun Liu