Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen.
Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen.
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DOI：
10.1007/s10029-020-02315-2
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Klinge U
中科院分区：
文献类型：
--
作者：
Dievernich A;Achenbach P;Davies L;Klinge U
网状植入物广泛用于加固腹壁，尽管界面处不可避免的炎症异物反应（FBR）会导致并发症。巨噬细胞被怀疑调节随后的疤痕形成，但目前尚不清楚伴随胶原沉积的充分纤维疤痕形成是否主要取决于 M1 或 M2 巨噬细胞的存在。本研究调查了 7 个人类聚丙烯网的 FBR，由于主要症状是复发，这些网在中位合并时间 1 年后被移除。使用免疫荧光法，根据细胞密度、巨噬细胞 M1 (CD86) 和 M2 (CD163、CD206) 表型、I 型和 III 型胶原沉积以及作为胶原蛋白降解指标的基质金属蛋白酶 2 (MMP-2) 和 -8 的表达，在六个区域中对 FBR 进行检查，其中距网状纤维的距离增加至 350 µm。所有网状组织复合物都显示出细胞密度和巨噬细胞随着距网状纤维的距离而减少。总体而言，约 60% 的巨噬细胞呈现 M2 表型，而只有 6% 的巨噬细胞呈现 M1 表型。超过 70% 的巨噬细胞与胶原蛋白-I 或-III 共表达，超过 50% 的巨噬细胞与 MMP-2 共表达。聚丙烯网片的慢性 FBR 与 M2 巨噬细胞反应相关，伴有胶原沉积和 MMP-2 表达。这些发现挑战了 M1 巨噬细胞主要与炎症相关的观点，并强调将这些细胞极化为 M2 表型的医源性尝试可能不是改善长期异物反应的解决方案。本文的在线版本 (10.1007/s10029-020-02315-2) 包含补充材料，可供授权用户使用。
Mesh implants are widely used to reinforce the abdominal wall, although the inevitable inflammatory foreign body reaction (FBR) at the interface leads to complications. Macrophages are suspected to regulate the subsequent scar formation, but it is still unclear whether adequate fibrous scar formation with collagen deposition depends mainly on the presence of M1 or M2 macrophages. This study investigated the FBR to seven human polypropylene meshes, which were removed after a median incorporation time of 1 year due to the primary complaint of recurrence. Using immunofluorescence, the FBR was examined in six regional zones with increasing distance from the mesh fibers up to 350 µm, based on the cell densities, macrophage M1 (CD86) and M2 (CD163, CD206) phenotypes, deposition of collagen-I and -III, and expression of matrix metalloproteinase-2 (MMP-2) and -8 as indicator of collagen degradation. All mesh–tissue complexes demonstrated a decrease in cell density and macrophages with distance to the mesh fibers. Overall, about 60% of the macrophages presented an M2 phenotype, whereas only 6% an M1 phenotype. Over 70% of macrophages showed co-expression with collagen-I or -III and over 50% with MMP-2. The chronic FBR to polypropylene meshes is associated with an M2 macrophage response, which is accompanied by collagen deposition and MMP-2 expression. These findings challenge the idea that mainly M1 macrophages are related to inflammation and highlights that iatrogenic attempts to polarize these cells towards the M2 phenotype may not be a solution to ameliorate the long-term foreign body reaction. The online version of this article (10.1007/s10029-020-02315-2) contains supplementary material, which is available to authorized users.
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影响因子：
4
作者：
Krzyszczyk P;Schloss R;Palmer A;Berthiaume F
通讯作者：
Berthiaume F
影响因子：
6.4
作者：
Davies LC;Taylor PR
通讯作者：
Taylor PR
影响因子：
8.6
作者：
Pechkovsky, Dmitri V.;Prasse, Antje;Zissel, Gernot
通讯作者：
Zissel, Gernot
DOI：
10.1084/jem.176.1.287
发表时间：
1992-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Stein M;Keshav S;Harris N;Gordon S
通讯作者：
Gordon S
影响因子：
4.4
作者：
Schnoor, Michael;Cullen, Paul;Lorkowski, Stefan
通讯作者：
Lorkowski, Stefan