Fructan synthesis, accumulation, and polymer traits. I. Festulolium chromosome substitution lines.

Fructan synthesis, accumulation, and polymer traits. I. Festulolium chromosome substitution lines.
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DOI:
10.3389/fpls.2015.00486
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发表时间:
2015
影响因子:
5.6
通讯作者:
Turner LB
Turner LB
中科院分区:
生物学2区
文献类型:
--
作者:
Gallagher JA;Cairns AJ;Thomas D;Charlton A;Williams P;Turner LB

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在温带牧草中作为储存碳水化合物发现的果聚糖在再生和胁迫耐受性方面具有生理作用。它们对于新鲜和保存的牲畜饲料的营养价值也很重要,并且有可能用作生物精炼的原料。果聚糖含量的季节性变化和从头合成果聚糖的能力已经在羊茅单体取代系家族中进行了检查,以研究黑麦草-羊茅复合体中的草产生的聚合物的变化。黑麦草和羊茅之间存在显着差异。羊茅具有低聚合果聚糖含量和高低聚物/聚合物比率;从低聚物合成长度超过聚合度 6 (DP6) 的聚合物的速度很慢。然而,聚合物长度从 DP10/DP20 向上的延伸似乎相对自由地发生,并且与黑麦草不同,羊茅在 DP20 以上具有相对均匀的聚合物链长度扩展。这包括一些非常大的聚合物的存在。此外,在低源/高汇需求的条件下,羊茅保留了高浓度的果聚糖,包括聚合果聚糖和低聚果聚糖。有迹象表明,参与控制其中一些性状的主要基因可能位于羊茅的 3 号染色体上,从而为开发针对特定应用而优化的草提供了可能性。
The fructans found as storage carbohydrates in temperate forage grasses have a physiological role in regrowth and stress tolerance. They are also important for the nutritional value of fresh and preserved livestock feeds, and are potentially useful as feedstocks for biorefining. Seasonal variation in fructan content and the capacity for de novo fructan synthesis have been examined in a Festulolium monosomic substitution line family to investigate variation in the polymers produced by grasses in the ryegrass-fescue complex. There were significant differences between ryegrass and fescue. Fescue had low polymeric fructan content and a high oligomer/polymer ratio; synthesis of polymers longer than degree of polymerization 6 (DP6) from oligomers was slow. However, extension of polymer length from DP10/DP20 upward appeared to occur relatively freely, and, unlike ryegrass, fescue had a relatively even spread of polymer chain lengths above DP20. This included the presence of some very large polymers. Additionally fescue retained high concentrations of fructan, both polymeric and oligomeric, during conditions of low source/high sink demand. There were indications that major genes involved in the control of some of these traits might be located on fescue chromosome 3 opening the possibility to develop grasses optimized for specific applications.
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