A dynamic mechanical method to assess bulk viscoelastic behavior of the dentin extracellular matrix.
A dynamic mechanical method to assess bulk viscoelastic behavior of the dentin extracellular matrix.
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DOI：
10.1016/j.dental.2020.09.014
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Bedran-Russo AK
中科院分区：
文献类型：
--
作者：
Alania Y;Reis MCD;Nam JW;Phansalkar RS;McAlpine J;Chen SN;Pauli GF;Bedran-Russo AK
建立一种评价牙本质细胞外基质（ECM）体粘弹行为的方法，并评价诱导胶原交联与牙本质ECM粘弹性之间的关系。用诱导外源性胶原交联的试剂处理牙本质ECM：来自葡萄属葡萄的原花色素（PAC）- VVe、来自马尾松的PAC- PMe、戊二醛-（GA）或保持未处理（对照）。在处理时、用4 M尿素使蛋白质不稳定后以及在模拟体液中孵育7天、6个月和12个月后，在3点弯曲浸没夹中进行动态机械应变扫描法。计算Tan δ、储能（E '）、损耗（E”）和复合模量（E*），并使用双因素ANOVA和事后检验（α = 0.05）对数据进行统计学分析。使用ATR-FTIR光谱法评估蛋白质去稳定之前和之后的牙本质ECM的化学分析。对于所有粘弹性参数，发现研究因素（治疗与时间点，p < 0.001）之间存在显著相互作用。尽管去稳定后所有模量显著降低，但PAC处理的牙本质仍在统计学上高于对照组（p < 0.001），表明生物改性后永久性机械增强。钴交联的GA处理的牙本质不受去稳定化的影响（p = 0.873），并且在所有时间点显示出最低的阻尼能力（tan δ）（p < 0.001）。12个月后，PMe和VVe组的阻尼能力明显下降。所有酰胺IR共振的变化揭示了PAC介导的生物改性的部分化学逆转。粘弹性测量和红外光谱有助于阐明分子间胶原交联在牙本质ECM的机械行为中的作用。
To develop a protocol for assessment of the bulk viscoelastic behavior of dentin extracellular matrix (ECM), and to assess relationships between induced collagen cross-linking and viscoelasticity of the dentin ECM. Dentin ECM was treated with agents to induce exogenous collagen cross-linking: proanthocyanidins (PACs) from Vitis vinifera – VVe, PACs from Pinus massoniana - PMe, glutaraldehyde – (GA), or kept untreated (control). A dynamic mechanical strain sweep method was carried out in a 3-point bending submersion clamp at treatment; after protein destabilization with 4 M urea and after 7-day, 6-month, and 12-month incubation in simulated body fluid. Tan δ, storage (E’), loss (E”), and complex moduli (E*) were calculated and data were statistically analyzed using two-way ANOVA and post-hoc tests (α = 0.05). Chemical analysis of dentin ECM before and after protein destabilization was assessed with ATR-FTIR spectroscopy. Significant interactions between study factors (treatment vs. time points, p < 0.001) were found for all viscoelastic parameters. Despite a significant decrease in all moduli after destabilization, PAC-treated dentin remained statistically higher than control (p < 0.001), indicating permanent mechanical enhancement after biomodification. Covalently crosslinked, GA-treated dentin was unaffected by destabilization (p = 0.873) and showed the lowest damping capacity (tan δ) at all time points (p < 0.001). After 12 months, the damping capacity of PMe and VVe groups decreased significantly. Changes in all amide IR resonances revealed a partial chemical reversal of PAC-mediated biomodification. Viscoelastic measurements and IR spectroscopy aid in elucidating the role of inter-molecular collagen cross-linking in the mechanical behavior of dentin ECM.
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DOI：
10.1016/j.jmbbm.2011.05.003
发表时间：
2011-10
影响因子：
3.9
作者：
Castellan, Carina Strano;Bedran-Russo, Ana Karina;Karol, Sachin;Rodrigues Pereira, Patricia Nobrega
通讯作者：
Rodrigues Pereira, Patricia Nobrega
影响因子：
3.4
作者：
Hatami-Marbini, Hamed;Rahimi, Abdolrasol
通讯作者：
Rahimi, Abdolrasol
影响因子：
7.6
作者：
Macedo, G. V.;Yamauchi, M.;Bedran-Russo, A. K.
通讯作者：
Bedran-Russo, A. K.
影响因子：
2.9
作者：
CHEUNG, DT;PERELMAN, N;NIMNI, ME
通讯作者：
NIMNI, ME
影响因子：
5
作者：
Bedran-Russo, Ana K.;Pauli, Guido F.;Chen, Shao-Nong;McAlpine, James;Castellan, Carina S.;Phansalkar, Rasika S.;Aguiar, Thaiane R.;Vidal, Cristina M. P.;Napotilano, Jose G.;Nam, Joo-Won;Leme, Ariene A.
通讯作者：
Leme, Ariene A.