The importance of size-exclusion characteristics of type I collagen in bonding to dentin matrices.
The importance of size-exclusion characteristics of type I collagen in bonding to dentin matrices.
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DOI：
10.1016/j.actbio.2013.07.037
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发表时间：
2013-12
影响因子：
9.7
通讯作者：
Pashley, D. H.
中科院分区：
文献类型：
--
作者：
Takahashi, M.;Nakajima, M.;Tagami, J.;Scheffel, D. L. S.;Carvalho, R. M.;Mazzoni, A.;Cadenaro, M.;Tezvergil-Mutluay, A.;Breschi, L.;Tjaderhane, L.;Jang, S. S.;Tay, F. R.;Agee, K. A.;Pashley, D. H.
牙本质的矿物相主要位于胶原纤维内。在发育过程中，首先形成骨或牙本质胶原原纤维，然后原纤维内的水被磷灰石微晶取代。矿化胶原蛋白含有很少的水。在牙本质粘合过程中，矿化牙本质的酸蚀会溶解矿物质微晶并用水代替它们。在牙本质粘合的渗透阶段，粘合共聚单体应该用粘合单体取代所有的胶原水，然后聚合成共聚物。最近发表的一篇评论的作者认为，牙科单体太大，无法进入并取代胶原纤维中的水。如果这是真的，那么与牙本质胶原蛋白结合的内源性蛋白酶可能会导致胶原蛋白的无阻碍降解，从而导致树脂-牙本质键的耐久性差。目前的工作采用类似凝胶过滤的柱色谱技术，使用牙本质粉代替 Sephadex，研究了牙本质胶原蛋白的尺寸排阻特性。包括粘合剂单体在内的测试分子的洗脱体积显示，小于约1000 Da的粘合剂单体可以自由扩散到胶原水中，而10,000 Da的分子开始被排除，牛血清白蛋白（66,000 Da）被完全排除。这些结果验证了牙科单体在蚀刻和冲洗粘合剂渗透过程中可以渗透到胶原蛋白分子之间的概念。
The mineral phase of dentin is located primarily within collagen fibrils. During development, bone or dentin collagen fibrils are formed first and then water within the fibril is replaced with apatite crystallites. Mineralized collagen contains very little water. During dentin bonding, acid-etching of mineralized dentin solubilizes the mineral crystallites and replaces them with water. During the infiltration phase of dentin bonding, adhesive comonomers are supposed to replace all of the collagen water with adhesive monomers that are then polymerized into copolymers. The authors of a recently published review suggested that dental monomers were too large to enter and displace water from collagen fibrils. If that were true, the endogenous proteases bound to dentin collagen could be responsible for unimpeded collagen degradation that is responsible for the poor durability of resin-dentin bonds. The current work studied the size-exclusion characteristics of dentin collagen, using a gel-filtration-like column chromatography technique, using dentin powder instead of Sephadex. The elution volumes of test molecules, including adhesive monomers, revealed that adhesive monomers smaller than about 1000 Da can freely diffuse into collagen water, while molecules of 10,000 Da begin to be excluded, and bovine serum albumin (66,000 Da) was fully excluded. These results validate the concept that dental monomers can permeate between collagen molecules during infiltration by etch-and-rinse adhesives.
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影响因子：
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