Galectin-1 on cervical epithelial cells is a receptor for the sexually transmitted human parasite Trichomonas vaginalis.
Galectin-1 on cervical epithelial cells is a receptor for the sexually transmitted human parasite Trichomonas vaginalis.
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DOI：
10.1111/j.1462-5822.2008.01190.x
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发表时间：
2008-10
影响因子：
3.4
通讯作者：
Johnson PJ
中科院分区：
文献类型：
--
作者：
Okumura CY;Baum LG;Johnson PJ
细胞外原生动物寄生虫阴道毛滴虫引起最普遍的非病毒性性传播人类感染，但感染的发病机制尚不清楚，宿主细胞受体尚未描述。阴道绦虫的表面覆盖着一种称为脂磷聚糖（LPG）的糖缀合物，它在寄生虫对人体细胞的粘附和细胞毒性中起作用。阴道T. LPG含有大量的半乳糖，使这种多糖成为半乳糖结合凝集素家族的候选识别物。在这里，我们发现半乳糖凝集素-1 （gal-1）在人宫颈上皮细胞中表达，并结合阴道绦虫LPG。Gal-1以碳水化合物依赖的方式与寄生虫结合，在阴道绦虫LPG的存在下被抑制。将纯化的gal-1添加到宫颈上皮细胞中也能增强寄生虫的结合，而通过转染小干扰RNA （small interfering RNA, siRNA）降低gal-1的表达则会降低寄生虫的结合。相反，相关的半乳糖凝集素-7 （gal-7）不能以碳水化合物依赖的方式与阴道绦虫结合，也不能介导寄生虫附着到宿主细胞上。我们的数据与阴道绦虫存在多种宿主细胞受体一致，其中gal-1是第一个被鉴定出来的，并强调了糖缀合物在宿主-病原体相互作用中的重要性。
The extracellular protozoan parasite Trichomonas vaginalis causes the most prevalent non-viral sexually transmitted human infection, yet the pathogenesis of infection is poorly understood, and host cell receptors have not been described. The surface of T. vaginalis is covered with a glycoconjugate called lipophosphoglycan (LPG), which plays a role in the adherence and cytotoxicity of parasites to human cells. T. vaginalis LPG contains high amounts of galactose, making this polysaccharide a candidate for recognition by the galactose-binding galectin family of lectins. Here we show that galectin-1 (gal-1) is expressed by human cervical epithelial cells and binds T. vaginalis LPG. Gal-1 binds to parasites in a carbohydrate-dependent manner that is inhibited in the presence of T. vaginalis LPG. Addition of purified gal-1 to cervical epithelial cells also enhances parasite binding, while a decrease in gal-1 expression by small interfering RNA (siRNA) transfection decreases parasite binding. In contrast, the related galectin-7 (gal-7) does not bind T. vaginalis in a carbohydrate-dependent manner, and is unable to mediate attachment of parasites to host cells. Our data are consistent with the presence of multiple host cell receptors for T. vaginalis of which gal-1 is the first to be identified and highlight the importance of glycoconjugates in host–pathogen interactions.
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影响因子：
3.1
作者：
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通讯作者：
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通讯作者：
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