Immunoprotection Efficacy of Con A-Purified Proteins against Haemonchus contortus in Goats.
Immunoprotection Efficacy of Con A-Purified Proteins against Haemonchus contortus in Goats.
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刀豆蛋白 A 纯化蛋白对山羊捻转血矛线虫的免疫保护作用
DOI：
10.3390/vaccines10111891
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发表时间：
2022-11-09
期刊：
影响因子：
7.8
通讯作者：
Hu M
中科院分区：
文献类型：
--
作者：
Ye L;Zhang Y;Wu S;Wang Z;Liu F;Wang C;Hu M
寄生线虫是重要的动物病原体，在全球范围内造成重大的经济损失。目前的重复治疗已导致线虫种群中广泛的驱虫剂抗性，因此疫苗开发提供了一种替代控制方法。然而，只有一种有效的疫苗（名为Barbervax）已经开发出来，以保护动物免受最致病的反刍动物线虫之一-捻转血矛线虫（理发师的杆虫）。该疫苗含有一种主要成分，刀豆球蛋白A（Con A）纯化的H11抗原，已显示其在绵羊品种中诱导高水平（>85%）的免疫保护，但在山羊品种中，这种天然蛋白的免疫保护试验仍然缺乏。在这里，我们评估了低剂量Con A纯化蛋白对控制H.山羊的捻转感染。将4月龄波尔山羊等分为5 μg和10 μg天然蛋白质的两个接种组和一个佐剂对照组。每只山羊皮下免疫三次，然后用7000只感染性第三期幼虫（L3）进行攻击。对试验羊的粪虫卵数、贫血程度、血清抗体水平、皱胃粘膜抗体水平和蠕虫负荷进行了检测。结果表明，5 μg和10 μg免疫组均能有效地保护山羊，与佐剂对照组相比，平均FEC分别降低71.8%和68.6%，平均虫负荷分别降低69.8%和61.6%。此外，我们发现，血清抗体反应的Con A纯化的蛋白质占主导地位的IgG亚型，但粘膜抗体反应未检测到。这些数据表明，Con A纯化的蛋白质在山羊中诱导有效的免疫保护，并强调其控制这种广泛的寄生虫的意义。
Parasitic nematodes are important pathogens that infect animals, causing significant economic losses globally. Current repeated treatments have led to widespread anthelmintic resistance in nematode populations, so vaccine development offers an alternative control approach. However, only one effective vaccine (named Barbervax) has been developed to protect animals against one of the most pathogenic nematodes of ruminants—Haemonchus contortus (the barber’s pole worm). This vaccine contains a dominant component, Concanavalin A (Con A) purified H11 antigen, which has been shown to induce high levels (>85%) of immune protection in sheep breeds, but in goat breeds, the immunoprotection test of this native protein is still lacking. Here, we evaluated the protective efficacy of low-dose Con A-purified proteins for controlling the H. contortus infection in goats. Four-month-old Boer goats were equally divided into two vaccinated groups of 5 μg and 10 μg native proteins, and one adjuvant control. Each goat was immunized subcutaneously thrice and then challenged with 7000 infective third-stage larvae (L3s). The fecal egg count (FEC), degree of anemia, antibody levels of serum and abomasum mucosa, as well as worm burdens, were detected in experimental goats. Our results showed that both 5 μg and 10 μg vaccinated groups induced the effective protection in goats, reduced mean FEC by 71.8% and 68.6%, and mean worm burdens by 69.8% and 61.6%, respectively, compared to the adjuvant control. In addition, we detected that the serum antibody responses to the Con A-purified proteins were dominated by the IgG subtype, but the mucosal antibody responses were not detected. These data demonstrate Con A-purified proteins induced effective immunoprotection in goats, and underline their significance for controlling this widespread parasite.
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影响因子：
2.6
作者：
Mickiewicz, Marcin;Czopowicz, Michal;Kaba, Jaroslaw
通讯作者：
Kaba, Jaroslaw
影响因子：
2.1
作者：
Kalyanasundaram, Aravindan;Jawahar, Shabnam;Raman, Muthusamy
通讯作者：
Raman, Muthusamy
影响因子：
2.6
作者：
Benavides, Magda, V;Souza, Carlos J. H.;Moraes, Jose C. F.
通讯作者：
Moraes, Jose C. F.
影响因子：
2.6
作者：
Meier, Lorena;Torgerson, Paul R.;Hertzberg, Hubertus
通讯作者：
Hertzberg, Hubertus
DOI：
10.1073/pnas.1105200108
发表时间：
2011-09-20
影响因子：
11.1
作者：
Jewell, Christopher M.;Lopez, Sandra C. Bustamante;Irvine, Darrell J.
通讯作者：
Irvine, Darrell J.