Centralspindlin and chromosomal passenger complex behavior during normal and Rappaport furrow specification in echinoderm embryos.
Centralspindlin and chromosomal passenger complex behavior during normal and Rappaport furrow specification in echinoderm embryos.
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DOI：
10.1002/cm.21061
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发表时间：
2012-10
期刊：
影响因子：
2.9
通讯作者：
Shuster, Charles Bradley
中科院分区：
文献类型：
--
作者：
Argiros, Haroula;Henson, Lauren;Holguin, Christiana;Foe, Victoria;Shuster, Charles Bradley
关键词：
染色体乘客 (CPC) 和 Centralspindlin 复合体对于组织后期中央纺锤体并提供定位子核之间细胞因子沟槽的线索至关重要。然而，棘皮动物的受精卵也能够在背靠背定位的紫苑之间形成“Rappaport 沟”，而无需干预染色体。为了了解这些复合物如何促进正常和 Rappaport 沟的形成，我们研究了生存素和有丝分裂驱动蛋白样蛋白 1 (MKLP1)（分别是 CPC 和 Centralspindlin 复合物的成员）的定位模式，以及 CPC 抑制对单核和双核棘皮动物受精卵分裂的影响。在受精卵中，Survivin 最初定位于中期染色体，在后期开始时重新定位于中央纺锤体，然后与 MKLP1 一起以 Aurora 依赖性方式向赤道皮层扩散。极光激酶活性的抑制导致中央纺锤体组织的破坏和皱纹的消退，尽管星体微管伸长和皱纹的形成是正常的。在含有两个平行纺锤体的双核细胞中，MKLP1 和 Survivin 定位于前中期板的平面，但在不相关纺锤体极之间形成的二次分裂平面中未观察到，除非那里异常存在染色体。然而，次生沟对极光抑制敏感，表明极光激酶可能仍然有助于沟进入，附近没有染色体。我们的研究结果提供了将经典显微操作研究与当前对动物细胞沟纹规范的分子理解相协调的见解。
The chromosomal passenger (CPC) and Centralspindlin complexes are essential for organizing the anaphase central spindle and providing cues that position the cytokinetic furrow between daughter nuclei. However, echinoderm zygotes are also capable of forming “Rappaport furrows” between asters positioned back-to-back without intervening chromosomes. To understand how these complexes contribute to normal and Rappaport furrow formation, we studied the localization patterns of Survivin and mitotic-kinesin-like-protein1 (MKLP1), members respectively of the CPC and the Centralspindlin complex, and the effect of CPC inhibition on cleavage in mono- and binucleate echinoderm zygotes. In zygotes, Survivin initially localized to metaphase chromosomes, upon anaphase onset relocalized to the central spindle and then, together with MKLP1 spread towards the equatorial cortex in an Aurora-dependent manner. Inhibition of Aurora kinase activity resulted in disruption of central spindle organization and furrow regression, although astral microtubule elongation and furrow initiation were normal. In binucleate cells containing two parallel spindles MKLP1 and Survivin localized to the plane of the former metaphase plate, but were not observed in the secondary cleavage plane formed between unrelated spindle poles, except when chromosomes were abnormally present there. However, the secondary furrow was sensitive to Aurora inhibition, indicating that Aurora kinase may still contribute to furrow ingression without chromosomes nearby. Our results provide insights that reconcile classic micromanipulation studies with current molecular understanding of furrow specification in animal cells.
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