Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon.
Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon.
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DOI：
10.1186/1756-3305-7-267
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发表时间：
2014-06-11
影响因子：
3.2
通讯作者：
das Graças Vale Barbosa M
中科院分区：
文献类型：
--
作者：
Santana RA;Magalhães LK;Magalhães LK;Prestes SR;Maciel MG;da Silva GA;Monteiro WM;de Brito FR;de Aguiar Raposo Câmara Coelho LI;Barbosa-Ferreira JM;Guerra JA;Silveira H;das Graças Vale Barbosa M
亚马孙地区的恰加斯病被认为是一种新兴的人畜共患病，主要是离散型单位（DTU）TcI和TcIV。这些DTU负责与口腔传播有关的急性疾病病例。通过血清学调查发现了慢性病病例。然而，传播方式无法确定，也无法确定慢性疾病与特定T。cruzi DTU的。本研究的目的是描述巴西亚马逊州慢性恰加斯病患者的克氏锥虫特征。对36例血清学查加斯病阳性患者进行了血培养和异种诊断，这些患者参加了在亚马逊州马瑙斯市城乡地区进行的血清学调查。从用于异种诊断的锥蝽粪便中提取DNA，采用PCR方法扩增小外显子基因非转录间隔区和线粒体基因细胞色素氧化酶亚基II（COII），并进行测序。血培养和异种诊断在100%的样本中为阴性，然而，分子技术显示，在36份异种诊断粪便样本中的13份（36%）中，T。cruzi被表征为DTU TcI，并且在同一DTU内鉴定出不同的单倍型。DTU TcI主要与亚马孙地区的查加斯病急性病例有关，也是亚马孙州一个地区患者慢性感染的原因。
Chagas disease in the Amazon region is considered an emerging anthropozoonosis with a predominance of the discrete typing units (DTUs) TcI and TcIV. These DTUs are responsible for cases of acute disease associated with oral transmission. Chronic disease cases have been detected through serological surveys. However, the mode of transmission could not be determined, or any association of chronic disease with a specific T. cruzi DTU’s. The aim of this study was to characterize Trypanosoma cruzi in patients with chronic Chagas disease in the State of Amazonas, Brazil. Blood culture and xenodiagnosis were performed in 36 patients with positive serology for Chagas disease who participated in a serological survey performed in urban and rural areas of Manaus, Amazonas. DNA samples were extracted from the feces of triatomines used for xenodiagnosis, and the nontranscribed spacer of the mini-exon gene and the mitochondrial gene cytochrome oxidase subunit II (COII) were amplified by PCR and sequenced. Blood culture and xenodiagnosis were negative in 100% of samples; however, molecular techniques revealed that in 13 out of 36 (36%) fecal samples from xenodiagnosis, T. cruzi was characterized as the DTU TcI, and different haplotypes were identified within the same DTU. The DTU TcI, which is mainly associated with acute cases of Chagas disease in the Amazon region, is also responsible for chronic infection in patients from a region in the State of Amazonas.
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影响因子：
3.2
作者：
Munoz-Calderon, A.;Diaz-Bello, Z.;Thomas, M. C.
通讯作者：
Thomas, M. C.
影响因子：
3.7
作者：
Monteiro WM;Magalhães LK;de Sá AR;Gomes ML;Toledo MJ;Borges L;Pires I;Guerra JA;Silveira H;Barbosa Md
通讯作者：
Barbosa Md
影响因子：
3.8
作者：
Monteiro WM;Margioto Teston AP;Gruendling AP;dos Reis D;Gomes ML;de Araújo SM;Bahia MT;Magalhães LK;de Oliveira Guerra JA;Silveira H;Toledo MJ;Vale Barbosa Md
通讯作者：
Vale Barbosa Md
DOI：
10.1590/s0037-86822011000600009
发表时间：
2011-12-01
影响因子：
2
作者：
Magalhães, Belisa Maria Lopes;Coelho, Leíla Ines Aguiar Raposo Câmara;Barbosa, Maria das Graças Vale
通讯作者：
Barbosa, Maria das Graças Vale
影响因子：
3.3
作者：
Mantilla, Julio C.;Zafra, German A.;Isabel Gonzalez, Clara
通讯作者：
Isabel Gonzalez, Clara