Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
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DOI：
10.1371/journal.pntd.0002069
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发表时间：
2013
影响因子：
3.8
通讯作者：
Vale Barbosa Md
中科院分区：
文献类型：
--
作者：
Monteiro WM;Margioto Teston AP;Gruendling AP;dos Reis D;Gomes ML;de Araújo SM;Bahia MT;Magalhães LK;de Oliveira Guerra JA;Silveira H;Toledo MJ;Vale Barbosa Md
在巴西亚马逊地区，恰加斯病的临床和流行病学框架与地方性典型传播区非常不同，这可能是由于流通的克氏锥虫种群的遗传和生物特征。26 T在瑞士小鼠中比较研究了来自西亚马逊地区的归因于TcI和TcIV DTU的cruzi种群，以检验T. cruzi克隆结构对其生物学和医学特性具有重大影响。测定了14 T感染小鼠的17项指标。11株为TcIV型。与TcI相比，TcIV原液促进了显著更短的开放前期（p<0.001）、更长的开放期（p<0.001）、更高的每日平均寄生虫血症（p = 0.009）和最大寄生虫血症（p = 0.015）值、更早的最大寄生虫血症（p<0.001）和死亡率（p = 0.018）的天数、更高的急性期死亡率（p = 0.047）、更高的感染率（p = 0.002）、更高的新鲜血液检查阳性率（p<0.001）、更高的慢性期早期ELISA阳性率（p= 0.022）和更高的慢性期晚期ELISA阳性率（p = 0.003）。 另一方面，与TcIV相比，TcI在早期慢性期显示出更高的死亡率值（p = 0.014），在任何器官中具有炎症过程的小鼠的频率更高（p = 0.005），在任何器官中具有组织寄生虫的小鼠的频率更高（p = 0.027），并且对苄硝唑的敏感性更高（p = 0.002）。 生存分析显示，从接种的一天到专利期的开始所经过的时间是显着较短的TcIV菌株和TcI感染后引发的死亡事件发生显着较晚的TcIV。值得注意的例外情况来自血培养和PCR中的阳性，其结果相似。 T.来自巴西亚马逊的属于TcI和TcIV DTU的cruzi种群在小鼠的生物学和医学特性方面是不同的。查加斯病是由原生动物寄生虫克氏锥虫引起的，在美洲大陆构成一个重要的健康问题。在巴西亚马逊地区，恰加斯病被认为是一个新出现的问题。很少有研究探讨的遗传和生物学框架的股票T。cruzi的研究，在巴西西部亚马逊地区，恰加斯病的发病率和死亡率较低，主要以慢性潜伏形式出现。本文对T.属于TcI和TcIV DTU的cruzi分离物，来自巴西亚马逊西部的亚马逊州。T.来自巴西亚马逊的属于TcI和TcIV DTU的cruzi种群在小鼠中的生物学和医学特性方面是不同的，如若干生物学参数所揭示的，后一种DTU具有更高的毒力。结果有力地支持了生物学差异与DTU之间的进化分歧成正比的工作假设，并强调需要考虑T.在涉及南美锥虫病临床多样性、免疫学、诊断、预后以及药物和疫苗试验的所有应用研究中，使用在南美锥虫病紧急地区流通的cruzi天然种群。
In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon Region attributed to the TcI and TcIV DTUs were comparatively studied in Swiss mice to test the hypothesis that T. cruzi clonal structure has a major impact on its biological and medical properties. Seventeen parameters were assayed in mice infected with 14 T. cruzi strains belonging to DTU TcI and 11 strains typed as TcIV. In comparison with TcI, TcIV stocks promoted a significantly shorter pre-patent period (p<0.001), a longer patent period (p<0.001), higher values of mean daily parasitemia (p = 0.009) and maximum of parasitemia (p = 0.015), earlier days of maximum parasitemia (p<0.001) and mortality (p = 0.018), higher mortality rates in the acute phase (p = 0.047), higher infectivity rates (p = 0.002), higher positivity in the fresh blood examination (p<0.001), higher positivity in the ELISA at the early chronic phase (p = 0.022), and a higher positivity in the ELISA at the late chronic phase (p = 0.003). On the other hand TcI showed higher values of mortality rates in the early chronic phase (p = 0.014), higher frequency of mice with inflammatory process in any organ (p = 0.005), higher frequency of mice with tissue parasitism in any organ (p = 0.027) and a higher susceptibility to benznidazole (p = 0.002) than TcIV. Survival analysis showing the time elapsed from the day of inoculation to the beginning of the patent period was significantly shorter for TcIV strains and the death episodes triggered following the infection with TcI occurred significantly later in relation to TcIV. The notable exceptions come from positivity in the hemocultures and PCR, for which the results were similar. T. cruzi stocks belonging to TcI and TcIV DTUs from Brazilian Amazon are divergent in terms of biological and medical properties in mice. Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, constituting an important health problem in the American Continent. In the Brazilian Amazon, Chagas disease has been recognized as an emerging problem. There are few studies exploring the genetic and biological framework of stocks of T. cruzi from the Western Brazilian Amazon, where Chagas disease has a profile of lower morbidity and mortality, appearing mainly in the chronic latent form. Here, we carried out the biological characterization in mice of T. cruzi isolates belonging to TcI and TcIV DTUs from the State of Amazonas, Western Brazilian Amazon. T. cruzi stocks belonging to TcI and TcIV DTUs from Brazilian Amazon are divergent in terms of biological and medical properties in mice, with a higher virulence for the latter DTU as revealed by several biological parameters. Results strongly support the working hypothesis that biological differences are proportional to the evolutionary divergence among the DTUs, and highlight the need to take into account the phylogenetic diversity of T. cruzi natural stocks circulating in the emergent areas for Chagas disease in all applied studies dealing with clinical diversity of Chagas disease, immunology, diagnosis, prognosis, and drug and vaccine trials.
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影响因子：
2
作者：
Florez, Oscar;Esper, Jhonatan;Gonzalez Rugeles, Clara Isabel
通讯作者：
Gonzalez Rugeles, Clara Isabel
影响因子：
64.8
作者：
Gaunt, MW;Yeo, M;Miles, MA
通讯作者：
Miles, MA
DOI：
10.1016/0035-9203(92)90156-7
发表时间：
1992-11-01
影响因子：
2.2
作者：
ANDRADE, SG;RASSI, A;LUQUETTI, AO
通讯作者：
LUQUETTI, AO
影响因子：
3.3
作者：
Añez, N;Crisante, G;Teixeira, MMG
通讯作者：
Teixeira, MMG
DOI：
10.1590/s0037-86822010000200013
发表时间：
2010-03-01
影响因子：
2
作者：
Brum-Soares, Lucia Maria;Xavier, Sergio Salles;Coura, Jose Rodrigues
通讯作者：
Coura, Jose Rodrigues