A novel degradation mechanism of naphthenic acids by marine Pseudoalteromonas sp.
A novel degradation mechanism of naphthenic acids by marine Pseudoalteromonas sp.
复制标题
海洋假交替单胞菌降解环烷酸的新机制。
DOI：
10.1016/j.jhazmat.2021.127534
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发表时间：
2021-10
影响因子：
13.6
通讯作者：
Yingxue Cai
中科院分区：
文献类型：
--
作者：
Shuaijun Zan;Jing Wang;Fengbo Wang;Zelong Li;Miaomiao Du;Yingxue Cai
环烷酸（Naphthenic Acids，NAs）是一种持久性有毒有机污染物，广泛存在于世界各地的环境中，对生态系统和公众健康造成严重威胁。然而，知识的行为和命运的NAs在海洋环境中仍然是未知的。研究了一株常见的海洋假交替单胞菌（Pseudoalteromonas sp.）对环己基乙酸（CHAA）的降解机理。结果表明，CHAA在好氧条件下可以完全降解，但在厌氧条件下不能直接利用。有趣的是，转录组和关键酶活性的结果表明，在好氧条件下诱导的CHAA降解途径仍然可以在厌氧条件下工作。该降解过程由乙酰辅酶A转移酶激活，在相关酶的协助下依次生成相应的环己烯、醇和酮，最后被羟甲基戊二酸辅酶A裂解酶裂解。此外，趋化性与好氧降解基因之间存在正相关（r = 0.976，P < 0.05），趋化性会促进细菌的运动和NAs的降解。细菌可以易位到NAs中，完成从好氧环境到厌氧环境的生物降解，这是NAs厌氧降解的一条新途径。该研究为NAs和其他有机污染物在海洋环境中的归宿提供了新的见解。
Naphthenic acids (NAs) are a persistent toxic organic pollutant that occur in different environment worldwide and cause serious threat to the ecosystem and public health. However, knowledge on the behavior and fate of NAs in marine environments still remains unknown. In this study, the degradation mechanism of NAs (cyclohexylacetic acid, CHAA) was investigated using an common indigenous marine Pseudoalteromonas sp. The results showed that CHAA could be degraded completely under aerobic condition, but could not be utilized directly under anaerobic condition. Interestingly, transcriptome and key enzyme activity results showed the CHAA degradation pathway induced under aerobic condition could still work in anaerobic condition. The degradation was activated by acetyl-CoA transferase and sequentially formed the corresponding cyclohexene, alcohol, and ketone with the assistance of related enzymes, and finally cleaved by hydroxymethylglutarate-CoA lyase. Besides, there was a positive correlation between chemotaxis and aerobic degradation genes (r = 0.976,P< 0.05), the chemotaxis would enhance bacterium movement and NAs biodegradation. It is proposed that bacterium could translocate to NAs and accomplish biodegradation from aerobic to anaerobic environments, which was a new anaerobic degradation pathway of NAs. This study provides new insights into the fate of NAs and other organic contaminants in marine environment.
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影响因子：
11.4
作者：
Y. Wan;Beili Wang;J. Khim;Seongjin Hong;W. Shim;Jianying Hu
通讯作者：
Y. Wan;Beili Wang;J. Khim;Seongjin Hong;W. Shim;Jianying Hu
影响因子：
64.8
作者：
J. Steele
通讯作者：
J. Steele
影响因子：
3.2
作者：
H. Ougham;P. Trudgill
通讯作者：
H. Ougham;P. Trudgill
影响因子：
2.7
作者：
V. Tremaroli;S. Fedi;S. Tamburini;C. Viti;E. Tatti;H. Ceri;R. Turner;D. Zannoni
通讯作者：
V. Tremaroli;S. Fedi;S. Tamburini;C. Viti;E. Tatti;H. Ceri;R. Turner;D. Zannoni
影响因子：
4.2
作者：
Gordillo, Felipe;Chavez, Francisco P.;Jerez, Carlos A.
通讯作者：
Jerez, Carlos A.